Related Experiment Video
Updated: Jun 12, 2025
Non-Invasive PET/MR Imaging in an Orthotopic Mouse Model of Hepatocellular Carcinoma
Published on: August 31, 2022
Protocol for establishing liver tumors via ultrasound-guided intrahepatic injection prior partial hepatic resection
Shadin Ghabra1, Rajiv Trehan2, Francisco Rodriguez-Matos2
1Thoracic and Gastrointestinal Malignancies Branch, Center for Cancer Research (CCR), National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD 20892, USA; Surgical Oncology Program, CCR, NCI, NIH, Bethesda, MD 20892, USA.
Abstract:
We present a protocol to generate a liver tumor murine model utilizing orthotopic ultrasound-guided intrahepatic injections of tumor cells and a partial hepatectomy. We describe cell and animal preparation and ultrasound-guided intrahepatic injections. We then depict how to conduct a partial hepatectomy addressing solutions to potential problems. This approach introduces a rapid and reproducible orthotopic implantation model of liver tumor model in a minimally invasive manner, allowing the surgical procedure to be optimized with minimal intra-abdominal injury. For complete details on the use and execution of this protocol, please refer to Brown et al.1.
