Related Experiment Video

Updated: Jun 9, 2025

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model
06:40

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

Published on: November 17, 2018

10.9K

Dietary pro-oxidant therapy by a vitamin K precursor targets PI 3-kinase VPS34 function

Manojit M Swamynathan1,2, Shan Kuang1, Kaitlin E Watrud1

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11771, USA.

Science (New York, N.Y.)
|October 24, 2024
PubMed

Insights

Pro-oxidant vitamin K precursor menadione sodium bisulfite (MSB) suppresses prostate cancer progression in mice. MSB also improved muscle function and lifespan in a myotubular myopathy model.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Oncology

Background:

  • Antioxidant vitamin E supplements are linked to increased prostate cancer (PC) risk in men.
  • The protective effects of pro-oxidants against PC remain largely unexplored.

Purpose of the Study:

  • To investigate the potential of pro-oxidant vitamin K precursor, menadione sodium bisulfite (MSB), in suppressing PC progression.
  • To elucidate the mechanism of action of MSB in cancer cells and its therapeutic potential in other diseases.

Main Methods:

  • Administration of MSB to mice with induced prostate cancer.
  • Investigating the interaction of MSB with the class III phosphatidylinositol 3-kinase VPS34.
  • Testing MSB in a mouse model of myotubular myopathy caused by MTM1 loss.

Main Results:

  • MSB suppressed prostate cancer progression in mice, inducing oxidative cell death.
  • MSB was found to antagonize VPS34 through oxidation of key cysteines, indicating a redox checkpoint in sorting.
  • Dietary MSB improved muscle histology, function, and lifespan in the myotubular myopathy model.

Conclusions:

  • Pro-oxidant MSB demonstrates therapeutic potential against prostate cancer and myotubular myopathy.
  • The findings highlight a redox checkpoint in cellular sorting regulated by VPS34.
  • Understanding pro-oxidant selectivity opens new avenues for therapeutic development.

More Related Videos

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases08:57

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

10.0K
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ10:05

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Published on: May 8, 2020

1.9K

Related Concept Videos

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
12.0K
Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...
2
Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
11.1K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.4K