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Updated: Jun 9, 2025
3' End Sequencing Library Preparation with A-seq2
Published on: October 10, 2017
iSeq: an integrated tool to fetch public sequencing data
Haoyu Chao1, Zhuojin Li2, Dijun Chen2
1Department of Bioinformatics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Motivation:
High-throughput sequencing technologies [next-generation sequencing (NGS)] are increasingly used to address diverse biological questions. Despite the rich information in NGS data, particularly with the growing datasets from repositories like the Genome Sequence Archive (GSA) at NGDC, programmatic access to public sequencing data and metadata remains limited.
Results:
We developed iSeq to enable quick and straightforward retrieval of metadata and NGS data from multiple databases via the command-line interface. iSeq supports simultaneous retrieval from GSA, SRA, ENA, and DDBJ databases. It handles over 25 different accession formats, supports Aspera downloads, parallel downloads, multi-threaded processes, FASTQ file merging, and integrity verification, simplifying data acquisition and enhancing the capacity for reanalyzing NGS data.
Availability And Implementation:
iSeq is freely available on Bioconda (https://anaconda.org/bioconda/iseq) and GitHub (https://github.com/BioOmics/iSeq).
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