Dual lateral flow test for simple and rapid simultaneous immunodetection of bisphenol A and dimethyl phthalate, two...

Contamination of the environment by technogenic endocrine disrupting compounds (EDCs) becomes serious threat to public health. To effectively prevent this threat, it is necessary to improve analytical methods for EDCs to ensure mass, fast and productive monitoring. In the given work, a dual lateral flow test (LFT) is developed in the first time for simultaneous immunodetection of bisphenol A and dimethyl phthalate, priority EDCs releasing from plastic and belonging to different chemical classes. It combines integrated detection of two EDCs by one analytical system with rapidity and simplicity of LFTs allowing for off-lab testing without additional reagents and devices. Gold nanoparticles differing in shape and color (red gold nanospheres and blue gold nanoflowers) are applied as markers to simplify interpretation of the obtained results. Under the optimal conditions chosen for efficient control of the both analytes, the detection limits of bisphenol A and dimethyl phthalate are 0.67 ng/mL and 2.22 ng/mL, respectively. Time of the assay is 15 min. The proposed dual LFT has confirmed its practical applicability by analyzing natural water samples with recovery of bisphenol A and dimethyl phthalate in the ranges of 90.4-107.0% and 86.8-118.0%, respectively.