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Isolation And Characterization Of Arsenic-tolerable Bacteria From Groundwater And Their Implementation On Rice Seedling's Shoot And Root Enhancement.

Home
Research Domains
Biological Sciences
Plant Biology
Phycology (incl. Marine Grasses)
Isolation And Characterization Of Arsenic-tolerable Bacteria From Groundwater And Their Implementation On Rice Seedling's Shoot And Root Enhancement.

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Isolation and Characterization of Arsenic-Tolerable Bacteria from Groundwater and Their Implementation on Rice Seedling's Shoot and Root Enhancement.

Ashutosh Kabiraj¹, Urmi Halder¹, Rajib Bandopadhyay²

¹Department of Botany, The University of Burdwan, Golapbag, Purba Bardhaman, West Bengal, 713104, India.

Current Microbiology

|October 24, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Seven Bacillus species isolated from arsenic-contaminated groundwater exhibit resistance to heavy metals and antibiotics. These plant growth-promoting bacteria (PGPB) detoxify arsenic and enhance rice growth, suggesting their potential as bio-fertilizers in contaminated fields.

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Area of Science:

Environmental Microbiology
Plant Pathology
Agricultural Science

Background:

Arsenic contamination in soil negatively impacts plant metabolism and crop yield.
Arsenic-resistant plant growth-promoting bacteria (PGPB) can mitigate arsenic's detrimental effects by producing plant hormones.
Arsenic stress poses a significant threat to global food security, necessitating innovative solutions.

Purpose of the Study:

To isolate and characterize arsenic-resistant Bacillus species from arsenic-contaminated groundwater.
To evaluate the plant growth-promoting and arsenic-detoxifying capabilities of these bacterial isolates.
To assess the potential of these Bacillus species as bio-fertilizers for crops grown in arsenic-contaminated soils.

Main Methods:

Isolation of bacterial species from arsenic-contaminated groundwater in West Bengal, India.

Determination of tolerance levels to arsenate (AsV) and arsenite (AsIII) salts and various heavy metals.

Assessment of antibiotic susceptibility profiles.

Evaluation of plant growth-promoting traits such as nitrogen fixation, phosphate solubilization, and production of indole-3-acetic acid (IAA) and gibberellic acid (GA).

Testing the efficacy of isolates in enhancing rice seedling growth under arsenic stress.

Main Results:

Seven Bacillus species were isolated, demonstrating high resistance to arsenate (>3.12 g/L) and arsenite (>0.65 g/L).
Isolates exhibited resistance to multiple heavy metals (Cu2+, Fe2+, Co2+, Zn2+, Pb2+) but were susceptible to certain antibiotics.
All species showed arsenite detoxification capabilities and produced plant growth-promoting substances (IAA, GA, proline).
Most Bacillus isolates significantly enhanced rice seedling root and shoot lengths under arsenic stress.

Conclusions:

The isolated Bacillus species possess significant arsenic resistance and plant growth-promoting attributes.
These bacteria can detoxify arsenite and support plant growth under arsenic stress conditions.
Bacillus spp. show promise as effective bio-fertilizers for agricultural applications in arsenic-contaminated environments.