Evaluation of Antioxidant Effects of Coenzyme Q10 against Hyperglycemia-Mediated Oxidative Stress by Focusing on Nrf2/Keap1/HO-1 Signaling Pathway in the Liver of Diabetic Rats
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View abstract on PubMed
Summary
This summary is machine-generated.Coenzyme Q10 (CoQ-10) supplementation ameliorates liver oxidative stress in diabetic rats by modulating the Nrf2/Keap1/ARE pathway. This study demonstrates CoQ-10
Area Of Science
- Biochemistry
- Molecular Biology
- Pharmacology
Background
- Hyperglycemia in diabetes mellitus causes oxidative stress, leading to liver damage and complications.
- Coenzyme Q10 (CoQ-10) is known to reduce oxidative stress, but its precise mechanisms in diabetes are not fully understood.
- The Nrf2/Keap1/ARE signaling pathway plays a crucial role in cellular antioxidant defense.
Purpose Of The Study
- To investigate the antioxidant effects of CoQ-10 on hyperglycemia-induced oxidative stress in the livers of diabetic rats.
- To elucidate the molecular mechanisms underlying CoQ-10's protective effects, focusing on the Nrf2/Keap1/ARE pathway.
- To evaluate the impact of CoQ-10 on key oxidative stress markers and gene expression in diabetic rat livers.
Main Methods
- Thirty male Wistar rats were divided into five groups: control, sesame oil, CoQ-10 treated, diabetic, and diabetic+CoQ-10 treated.
- Diabetes was induced, and treatments were administered for six weeks.
- Liver tissue was analyzed for oxidative stress markers (malondialdehyde, catalase, glutathione peroxidase, glutathione) and gene expression (Nrf2, Keap1, HO-1, NQO1) using spectrophotometry and real-time PCR.
Main Results
- Diabetes significantly increased Keap1 expression and decreased Nrf2, HO-1, and NQO1 gene expression.
- Diabetic rats exhibited reduced antioxidant enzyme activity and glutathione levels, alongside increased malondialdehyde.
- CoQ-10 supplementation normalized Nrf2/Keap1/HO-1/NQO1 gene expression, reduced malondialdehyde, and increased antioxidant capacity in diabetic rats.
Conclusions
- Coenzyme Q10 (CoQ-10) demonstrates significant antioxidant properties in the context of diabetic liver injury.
- CoQ-10 modulates the Nrf2/Keap1/HO-1/NQO1 signaling pathway, enhancing the liver's antioxidant defense system.
- These findings suggest CoQ-10 as a potential therapeutic agent for managing diabetes-related liver complications.
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