Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

8.5K
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
8.5K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.7K
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.2K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.2K
Mass Spectrometry of Amines01:19

Mass Spectrometry of Amines

4.1K
In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule: a molecule with an odd number of nitrogen atoms produces a parent ion with an odd molecular weight. The remaining fragments have an even mass.
Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit...
4.1K
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

1.4K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
1.4K
Mass Spectrometry: Amine Fragmentation00:55

Mass Spectrometry: Amine Fragmentation

1.5K
Amines can be identified using mass spectroscopy based on their characteristic fragmentation patterns. The molecular ions of amines undergo fragmentation via ⍺-cleavage. The ⍺-cleavage of the carbon-carbon bonds in amines generates an alkyl radical and resonance-stabilized nitrogen-containing cation.
In amines, the number of nitrogen atoms affects the mass of the molecular ion, which is described by the nitrogen rule of mass spectrometry. This rule states that a compound containing...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mamba-YOLO-SRC: An Automatic Deep Learning Framework for Respiratory Behavior Detection in the Chinese Giant Salamander.

Animals : an open access journal from MDPI·2026
Same author

[Contamination Characteristics and Source Apportionment of Heavy Metals in Soil around the Agricultural Land in Coal Mining Concentration Areas Based on APCS-MLR and PMF Models].

Huan jing ke xue= Huanjing kexue·2026
Same author

Structural and dynamic properties of the YTH domain in complex with N<sup>6</sup>-methyladenosine RNA studied by accelerated molecular dynamics simulations.

Quantitative biology (Beijing, China)·2026
Same author

Small-molecule modulators of the androgen receptor N-terminal domain: Advances in medicinal chemistry for prostate cancer.

European journal of medicinal chemistry·2026
Same author

Disassembly orders of protein complexes by coarse-grained simulations with dynamic native contacts.

Biophysical journal·2025
Same author

Quantitative Real-Time Fluorine NMR Spectroscopy for Enzyme Kinetics: Hydrolysis of N-Trifluoroacetylglycine (TFAG) by Acylase I.

The journal of physical chemistry. B·2025

Related Experiment Video

Updated: Jun 9, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.4K

Targeted F 19 - tags to detect amino acids in complex mixtures using NMR spectroscopy.

Keeton Montgomery1, Aya Elhabashy1, Guanglin Chen1

  • 1Department of Chemistry and Biochemistry, California State University, Fresno, CA 93740, USA.

Journal of Fluorine Chemistry
|October 25, 2024
PubMed
Summary

Fluorine-19 Nuclear Magnetic Resonance (19F NMR) can identify amino acids by measuring reaction kinetics. This technique offers a powerful, yet underutilized, method for chemical analysis and detection in complex samples.

Keywords:
Amino acidFluorine-19KineticsMixture analysisNuclear Magnetic Resonance (NMR)

More Related Videos

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition
07:40

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Published on: May 17, 2024

1.2K
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
13:59

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Published on: December 12, 2013

6.2K

Related Experiment Videos

Last Updated: Jun 9, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.4K
Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition
07:40

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Published on: May 17, 2024

1.2K
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
13:59

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Published on: December 12, 2013

6.2K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy

Background:

  • Fluorine-19 Nuclear Magnetic Resonance (19F NMR) offers high sensitivity and a broad spectral range.
  • Despite its advantages, 19F NMR is underutilized in educational and research settings.
  • Amino acid (AA) analysis is crucial in various scientific fields, including metabolomics.

Purpose of the Study:

  • To demonstrate the utility of 19F NMR for chemical kinetics investigations.
  • To explore the reaction kinetics between a specific tag and individual amino acids.
  • To establish a method for identifying and quantifying amino acids in complex mixtures using 19F NMR.

Main Methods:

  • Synthesis of the tag 2,5-dioxopyrrolidin-1-yl-2-(trifluoromethyl)benzoate.
  • Continuous measurement of reaction kinetics using 19F NMR in the presence of selected amino acids.
  • Analysis of reaction rate constants for tag-amino acid interactions.

Main Results:

  • Distinct reaction rate constants were observed for the interaction of the tag with individual amino acids.
  • Tag formation with amino acids was completed within 24-48 hours in aqueous solutions.
  • The observed kinetic differences suggest potential for amino acid identification.

Conclusions:

  • The study highlights the potential of 19F NMR for chemical kinetics studies.
  • The developed approach using a trifluoromethyl benzoate tag shows promise for amino acid detection and quantification.
  • This method could be valuable for applications in metabolomics and chemical education.