Related Experiment Video

Updated: Jun 9, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.6K

SNORD99 promotes endometrial cancer development by inhibiting GSDMD-mediated pyroptosis through 2'-O-methylation

Jing-Yuan Xian1,2, Wu Wu1,2, Xi Chen1,2

  • 1Department of Obstetrics and Gynecology, Guangzhou Key Laboratory of Targeted Therapy for Gynecologic Oncology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Journal of Cellular and Molecular Medicine
|October 25, 2024
PubMed

Insights

SNORD99, a small nucleolar RNA, promotes endometrial cancer by suppressing pyroptosis. It methylates GSDMD, inhibiting cell death and enhancing tumor growth, suggesting new therapeutic targets.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • Eukaryotic cells have self-destruction mechanisms like pyroptosis.
  • Small nucleolar RNAs (snoRNAs) can regulate gene expression and are implicated in cancer.
  • Pyroptosis is a pro-inflammatory programmed cell death pathway crucial in immunity and disease.

Purpose of the Study:

  • To investigate the role of SNORD99 in endometrial cancer (EC) pathogenesis.
  • To elucidate the molecular mechanism by which SNORD99 influences cell death pathways.
  • To explore SNORD99 as a potential therapeutic target for EC.

Main Methods:

  • StarBase and qRT-PCR for SNORD99 expression analysis in EC tissues.
  • Cell proliferation, migration assays, and ASO-mediated knockdown in EC cells.
  • RNA-sequencing, RNP complex formation analysis, RTL-P, Western blotting, and microscopy to study SNORD99-GSDMD interaction and pyroptosis.

Main Results:

  • SNORD99 was significantly upregulated in EC tissues and promoted EC cell migration and proliferation.
  • SNORD99 overexpression led to the formation of a SNORD99-FBL RNP complex and increased GSDMD 2'-O-methylation.
  • SNORD99 suppressed pyroptosis by reducing GSDMD, caspase-1, and NLRP3 protein levels, thereby promoting EC progression.

Conclusions:

  • SNORD99 promotes endometrial cancer progression by inhibiting pyroptosis through GSDMD 2'-O-methylation.
  • Targeting SNORD99 or enhancing pyroptosis could be a viable therapeutic strategy for EC.
  • This study reveals a novel mechanism linking snoRNAs to cell death regulation in cancer.
Keywords:
2'‐O‐methylationGSDMDendometrial cancerpyroptosissmall nucleolar RNA

More Related Videos

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.7K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.5K

Related Concept Videos

Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
148
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K