Evaluating a New Photopheresis System: A Comparison with Two Established Systems on Cell Yield and Collection
Orkan Kartal1, Sandra Laner-Plamberger1, Eva Rohde1,2
1Department for Transfusion Medicine, University Hospital of Salzburg, Paracelsus Medical University Salzburg, Muellner Hauptstraße 48, 5020 Salzburg, Austria.
Diagnostics (Basel, Switzerland)
|October 25, 2024
Summary
The Amicus Blue system collects enough mononuclear cells for extracorporeal photopheresis (ECP), but has lower collection efficiency and yield compared to Therakos Cellex and Spectra Optia systems.
Area of Science:
- Cellular Therapy
- Immunology
- Medical Devices
Background:
- Extracorporeal photopheresis (ECP) is an established cell therapy for various diseases.
- Comparison of ECP systems is crucial for optimizing treatment efficacy.
Purpose of the Study:
- To compare the Amicus Blue inline system with Therakos Cellex inline and Spectra Optia offline systems.
- Evaluate collection efficiency, mononuclear cell (MNC) yield, and processing time.
Main Methods:
- Retrospective study of 127 Spectra Optia, 93 Amicus Blue, and 81 Therakos Cellex procedures.
- Flow cytometry analysis of MNCs for CD45 and CD14 expression.
Main Results:
- Therakos Cellex showed the highest MNC collection efficiency (74.42%).
- Spectra Optia yielded the most MNCs (42.69 ×10⁶/kg).
- Amicus Blue had significantly lower collection efficiency and MNC yield.
Conclusions:
- Amicus Blue collects sufficient MNCs for ECP but is less efficient than other systems.
- Spectra Optia offline system demonstrated superior MNC yield.
- This is the first direct comparison of Amicus Blue with established ECP systems.
