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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

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Effective Boundary Correction for Deterministic Lateral Displacement Microchannels to Improve Cell Separation: A

Shaghayegh Mirhosseini^1,2, Mohammadmahdi Eskandarisani^3,4, Aryanaz Faghih Nasiri¹

¹School of Electrical and Computer Engineering, College of Engineering, University of Tehran, Tehran 1439957131, Iran.

|October 25, 2024

Summary

This study enhances microfluidic cell separation using deterministic lateral displacement (DLD) with a novel boundary correction technique. This method significantly improves the efficiency of isolating circulating tumor cells (CTCs) and red blood cells.

Keywords:

boundary correction cell separation deterministic lateral displacement microfluidics

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Area of Science:

Biomedical Engineering
Microfluidics
Cell Separation Technology

Background:

Microfluidic devices are crucial for particle and cell separation.
Deterministic Lateral Displacement (DLD) offers size-based cell sorting.
Channel boundary effects negatively impact separation efficiency in microfluidic devices.

Purpose of the Study:

To design and fabricate a microfluidic device for efficient cell separation.
To investigate the impact of enhanced channel boundary structures on separation performance.
To improve the isolation of circulating tumor cells (CTCs) and red blood cells (RBCs).

Main Methods:

Fabrication of a microfluidic device utilizing deterministic lateral displacement (DLD).
Implementation of a novel boundary correction (BC) technique to optimize channel walls.
Quantitative analysis of cell separation efficiency using microscopic imaging and flow cytometry.

Main Results:

The DLD device with boundary correction achieved high separation efficiencies.
Circulating tumor cell (CTC) throughput exceeded 93%, and CTC isolation efficiency was over 89%.
Red blood cell (RBC) isolation efficiency surpassed 77%, while BC reduced separation efficiency by approximately 5%.

Conclusions:

The novel boundary correction technique significantly enhances microfluidic device performance.
This approach offers a promising solution for accurate and efficient cell separation in microchannels.
The developed device demonstrates high potential for clinical applications, particularly in cancer diagnostics.