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ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

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Paralytic Shellfish Toxin Extraction from Bivalve Meat for Analysis Using Potentiometric Chemical Sensors.

Ana Filipa R Cerqueira¹, Catarina Moreirinha¹, Mariana Raposo¹

¹Centre for Environmental and Marine Studies (CESAM) and Chemistry Department, University of Aveiro, 3810-193 Aveiro, Portugal.

|October 25, 2024

Summary

This summary is machine-generated.

A new method simplifies paralytic shellfish toxin (PST) detection in shellfish using chemical sensors. Acetic acid extraction with heating offers the best toxin recovery and sensor response, reducing the need for purification steps.

Keywords:

bivalves extraction paralytic shellfish toxins potentiometric sensors

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Area of Science:

Analytical Chemistry
Food Safety
Marine Biology

Background:

Paralytic shellfish toxins (PSTs) pose a significant risk to public health.
Accurate and efficient detection methods for PSTs in bivalve mollusks are crucial for food safety monitoring.
Existing methods often involve complex extraction and purification steps.

Purpose of the Study:

To develop a simple and reliable methodology for PST detection in bivalve tissues using potentiometric chemical sensors.
To evaluate and optimize PST extraction methods to minimize matrix effects and maximize toxin recovery.
To assess the necessity of extract purification for chemical sensor-based PST analysis.

Main Methods:

Evaluation of five PST extraction methods from mussel and oyster tissues.
Comparison of extraction efficiency and sensor response using potentiometric chemical sensors.
Utilized AOAC-recommended method as a reference for PST extraction.

Main Results:

Extraction using acetic acid with heating and water extraction yielded the highest responses from potentiometric chemical sensors.
Acetic acid extraction with heating provided the highest PST recovery from bivalve tissues.
Purification of extracts, typically required for LC-FLD, was found unnecessary for chemical sensor analysis.

Conclusions:

A simplified methodology for PST detection using potentiometric chemical sensors has been established.
Acetic acid extraction with heating is the preferred method for maximizing PST recovery and sensor performance.
Water extraction offers a rapid alternative, though with lower recovery rates; further research is needed to address matrix effects and improve sensor response.