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Updated: Jun 9, 2025

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
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The Sorting and Transport of the Cargo Protein CcSnc1 by the Retromer Complex Regulate the Growth, Development, and

Shuyuan Cheng1,2, Yunfei Long3, Xiaoyang Zhang4

  • 1College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.

Journal of Fungi (Basel, Switzerland)
|October 25, 2024
PubMed

Insights

The retromer complex is vital for transporting proteins in the fungus Corynespora cassiicola. This study shows retromer

Area of Science:

  • Mycology
  • Cell Biology
  • Molecular Biology

Background:

  • The retromer complex facilitates endosome-to-trans-Golgi network (TGN) transport in eukaryotes.
  • Research on retromer-mediated cargo protein transport in filamentous fungi, particularly concerning growth, development, and pathogenicity, is limited.

Purpose of the Study:

  • To investigate the role of the retromer complex in the pathogenic fungus Corynespora cassiicola.
  • To identify and characterize retromer cargo proteins involved in fungal infection.

Main Methods:

  • Transcriptome analysis to assess retromer gene expression during C. cassiicola invasion.
  • Gene knockout and complementation to determine the function of retromer components and cargo.
  • Subcellular localization studies using microscopy to pinpoint the location of the retromer complex and cargo proteins.
  • Co-immunoprecipitation to confirm interactions between retromer subunits and cargo proteins.

Main Results:

  • Expression of retromer complex genes (CcVPS35, CcVPS29, CcVPS26) was significantly upregulated during early C. cassiicola invasion.
  • The retromer complex localized to the vacuolar membrane, endosomes, and TGN.
  • The retromer core subunit CcVps35 interacts with the cargo protein CcSnc1.
  • CcSnc1, localized at the hyphal tip, endosomes, and Golgi, was mis-sorted to the vacuolar degradation pathway upon CcVPS35 deletion.
  • CcSnc1 is essential for C. cassiicola growth, development, and pathogenicity.

Conclusions:

  • The retromer complex mediates the sorting and transport of CcSnc1 from endosomes to the TGN in C. cassiicola.
  • This retromer-CcSnc1 pathway is crucial for the fungus's growth, development, and pathogenicity.
  • The findings highlight a key vesicular transport mechanism in fungal pathogenesis.
Keywords:
Corynespora cassiicolacargo sortingfungal developmentpathogenicityretromer

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