Related Experiment Video
Updated: Jun 9, 2025
11:26
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
13.6K
Identification of New Single Nucleotide Polymorphisms Potentially Related to Small Ruminant Lentivirus Infection
Magdalena Materniak-Kornas1, Katarzyna Ropka-Molik2, Katarzyna Piórkowska2
1Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland.
Pathogens (Basel, Switzerland)
|October 25, 2024
Summary
Small ruminant lentivirus (SRLV) infection control relies on identifying host genetic factors. Carpathian goats show specific gene variants, PARP14 and TMEM154, linked to SRLV resistance or susceptibility.
Area of Science:
- Veterinary Genetics
- Infectious Disease Epidemiology
- Animal Genomics
Background:
- Small ruminant lentivirus (SRLV) infections cause significant economic losses in sheep and goats globally.
- Current control strategies focus on eliminating infected animals, highlighting the need for alternative approaches like identifying genetic resistance factors.
- SRLV infection prevalence is high, with infected animals shedding the virus and spreading it within flocks.
Purpose of the Study:
- To investigate host genetic factors, specifically single nucleotide polymorphisms (SNPs), associated with small ruminant lentivirus (SRLV) proviral DNA load in Carpathian goats.
- To identify potential genetic markers for resistance or susceptibility to SRLV infection.
- To analyze the association between specific gene polymorphisms and SRLV infection status.
Main Methods:
- RNA-sequencing data from 12 SRLV-infected goats were used for variant calling to identify SNPs.
- Six SNPs within five genes (POU2AF1, BCAT2, TMEM154, PARP14, UBASH3A) were selected for genotyping in a cohort of 60 goats.
- Genotyping data were analyzed to determine the association between SNPs and the level of SRLV proviral DNA.
Main Results:
- The TT genotype of the PARP14 gene was exclusively observed in seronegative goats, suggesting a potential role in resistance.
- The CC genotype of the TMEM154 gene was found only in seropositive goats, indicating a possible association with susceptibility.
- Polymorphisms in POU2AF1 and UBASH3A genes were deemed less likely to be resistance/susceptibility markers due to potential deleterious effects on protein function.
Conclusions:
- The PARP14 and TMEM154 genes contain polymorphisms that may serve as valuable markers for assessing resistance or susceptibility to SRLV infection in goats.
- Identifying these genetic markers can aid in developing more targeted and effective SRLV control programs.
- Further research into the functional impact of these gene variants is warranted to fully understand their role in SRLV pathogenesis.
