A Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to...

Area of Science:

• Analytical Chemistry
• Food Science
• Biochemistry

Background:

• Polyphenols contribute to wine's sensory characteristics (color, astringency).
• Polyphenols possess antioxidant properties, offering potential health benefits.
• Accurate quantification of wine polyphenols is crucial for quality assessment and health studies.

Purpose of the Study:

• To develop a robust analytical method for quantifying a wide range of phenolic compounds in wine.
• To enable detailed phenolic profiling of red, rosé, and white wine varieties.
• To establish a versatile method adaptable for the detection of novel phenolic compounds.

Main Methods:

• Utilized ultra-high-performance liquid chromatography (UHPLC) coupled with a high-resolution mass spectrometry system (Orbitrap™).
• Employed a full-scan mass spectrometry approach for comprehensive compound detection.
• Achieved high selectivity and sub-parts per million (ppm) mass accuracy for precise quantification.

Main Results:

• Successfully quantified 90 distinct phenolic compounds in various wine types (red, rosé, white).
• Demonstrated high selectivity in analyzing complex wine matrices.
• The full-scan capability allowed for the simultaneous monitoring of numerous other compounds.

Conclusions:

• The developed UHPLC-Orbitrap™ method provides a powerful tool for detailed wine polyphenol analysis.
• The method's accuracy and adaptability make it suitable for both current research and future discoveries in wine chemistry.
• This approach enhances our understanding of wine composition and its associated health implications.