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Updated: Jun 9, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
MicroRNA Biogenesis, Gene Regulation Mechanisms, and Availability in Foods
Amilton S de Mello1, Bradley S Ferguson2, Erica L Shebs-Maurine1
1Department of Agriculture, Veterinary and Rangeland Sciences, University of Nevada, Reno 1664 N. Virginia St. Mail Stop 202, Reno, NV 89557, USA.
Abstract:
MicroRNAs (miRNAs) are small, non-coding RNAs that control gene expression by degrading or repressing mRNA translation into proteins. Research recently suggested that food-derived miRNAs are bioavailable and may be absorbed in the gastrointestinal tract (GIT). Since these small RNAs may reach the circulation and organs, possible interactions with host genes will lead to epigenetic effects that alter metabolism. Therefore, from a precision nutrition standpoint, exogenous miRNAs may be essential in modulating health status. This review summarizes the process of miRNA biogenesis, the post-translational mechanisms of gene regulation, and their bioavailability in animal- and plant-derived foods.
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