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Related Experiment Video

Updated: Jul 13, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

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Brevetoxin Aptamer Selection and Biolayer Interferometry Biosensor Application.

Bo Hu¹, Sheng-Qun Ouyang², Yu-Ping Zhu²

¹Naval Medical Center of PLA, Naval Medical University, Shanghai 200433, China.

|October 25, 2024

Summary

Researchers developed a novel aptasensor for detecting Brevetoxin-1 (PbTx-1), a potent marine neurotoxin. This biosensor offers a specific, reliable, and sensitive alternative to traditional methods for monitoring neurologic shellfish poisoning (NSP).

Keywords:

aptamer aptasensor biolayer interferometry brevetoxin

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Area of Science:

Marine Toxin Detection
Biosensor Technology
Molecular Recognition

Background:

Brevetoxins (PbTxs) are potent marine neurotoxins causing neurologic shellfish poisoning (NSP).
Increasing geographical distribution of PbTxs necessitates improved monitoring methods.
Current analysis methods face ethical and technical challenges.

Purpose of the Study:

To develop a novel aptasensor for the specific detection of Brevetoxin-1 (PbTx-1).
To address limitations of existing marine toxin detection techniques.
To provide a reliable alternative for regulatory monitoring.

Main Methods:

Concurrent aptamer selection for Brevetoxin-1 (PbTx-1) and Brevetoxin-2 (PbTx-2).
Construction of a biolayer interferometry (BLI) biosensor using a high-affinity PbTx-1 aptamer (A5-S3G).
Optimization of aptamer sequence through truncation and mutation for enhanced binding affinity.

Main Results:

Successfully selected DNA aptamers with high affinity and specificity for PbTx-1.
Developed a label-free BLI aptasensor with a detection range of 100-4000 nM and LOD of 4.5 nM for PbTx-1.
Demonstrated high specificity, no cross-reactivity with PbTx-2 or other marine toxins, and excellent stability in shellfish samples.

Conclusions:

The developed BLI aptasensor is a promising, innovative tool for specific and reliable PbTx-1 detection.
This aptasensor offers a viable alternative to traditional immunological methods for marine toxin monitoring.
The findings support the application of aptasensors in regulatory monitoring regimes for shellfish safety.