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Development of a New Tool to Detect Melampsora medusae f. sp. tremuloidae Causing Rust Disease on Populus tremuloides

Cécile Guinet1, Marius Buronfosse1, Philippe Tanguay2

  • 1ANSES Laboratoire de la Santé des Végétaux, Unité de Mycologie, USC INRAE 1480, Malzéville, France.

Plant Disease
|October 25, 2024
PubMed

Insights

A new real-time PCR assay can detect the quarantine fungus Melampsora medusae f. sp. tremuloidae. This method aids in monitoring the introduction and spread of this rust fungus, crucial for phytosanitary regulations.

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Mycology

Background:

  • Melampsora medusae f. sp. tremuloidae is a quarantine organism posing a risk to European aspen (Populus tremula).
  • This fungus causes rust disease on Populus tremuloides in North America.
  • The potential introduction and spread of this pathogen in Europe necessitates effective detection methods.

Purpose of the Study:

  • To develop a sensitive and specific PCR-based assay for detecting Melampsora medusae f. sp. tremuloidae.
  • To provide a tool for monitoring the phytosanitary risks associated with this quarantine organism.

Main Methods:

  • Development of a real-time PCR assay targeting the 28S rDNA gene.
  • Validation of the assay's sensitivity and specificity.

Main Results:

  • A reliable real-time PCR assay was successfully developed.
  • The assay demonstrated high sensitivity and specificity for detecting Melampsora medusae f. sp. tremuloidae.
  • The assay is compatible with various real-time PCR kits and platforms.

Conclusions:

  • The developed real-time PCR assay is a valuable tool for phytosanitary surveillance.
  • This method can effectively monitor the introduction and spread of Melampsora medusae f. sp. tremuloidae in Europe.
  • The assay supports regulatory efforts to prevent the establishment of this quarantine fungus.
Keywords:
Melampsora medusae f. sp. tremuloidaedetectionquarantine pest

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