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Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
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Published on: June 28, 2024

774

The liquid lectin array detects compositional glycocalyx differences using multivalent DNA-encoded lectins on phage.

Guilherme M Lima1, Zeinab Jame-Chenarboo1, Mirat Sojitra2

  • 1Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.

Cell Chemical Biology
|October 25, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a novel liquid lectin array (LiLA) using DNA-barcoded phages to detect subtle changes in cell surface glycans. This technology enables precise identification of disease biomarkers on cells in vitro and in vivo.

Keywords:
DNA barcodingLiLAcell surfaceglycan-binding proteinsglycocalyxlectin arraymultivalencyphageprofiling

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Detecting subtle glycocalyx changes is crucial for targeted therapies but challenging due to the lack of direct DNA/RNA correlation.
  • Current technologies struggle to identify minor glycan variations on cell surfaces.

Purpose of the Study:

  • To develop a method for sensitive and selective detection of disease-associated glycocalyx alterations.
  • To demonstrate the capability of multivalent lectin displays for glycan profiling.

Main Methods:

  • Utilized DNA-barcoded phages displaying multivalent lectin constructs (liquid lectin array - LiLA).
  • Engineered specific phage-lectin valencies (e.g., φ-CBM73) for differential glycan recognition.
  • Applied LiLA to distinguish between normal and cancer cells, and between cancer stem-like and non-stem populations.

Main Results:

  • LiLA constructs (φ-CBM73) showed non-linear ON/OFF recognition of sialoglycans on normal versus cancer cells.
  • Higher valency displays (φ-CBM290) or soluble proteins did not enhance sensitivity.
  • Multivalent displays successfully identified glycocalyx differences in cancer stem-like populations.

Conclusions:

  • Multivalent lectin displays on DNA-barcoded phages offer a powerful tool for detecting subtle glycocalyx differences.
  • LiLA technology enables in situ detection of minor glycan variations in vitro and in vivo, surpassing current limitations.