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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 9, 2025

Separation and Identification of Conventional Microplastics from Farmland Soils
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A strategy for quantifying microplastic particles in membrane filtration processes using flow cytometry.

Ryan J LaRue1, Samuel Koo1, Ashleigh Warren1

  • 1McMaster University Department of Chemical Engineering, Hamilton, ON, Canada.

Chemosphere
|October 25, 2024
PubMed
Summary
This summary is machine-generated.

Flow cytometry (FC) effectively quantifies microplastic (MP) concentrations in water. This study validates membrane filtration for MP removal, showing a 0.45 μm membrane achieves >99% rejection and a 5 μm membrane

Keywords:
Flow cytometryHumic acidMembranesMicrofiltrationMicroplasticsWastewater treatmentWater treatment

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Sampling, Sorting, and Characterizing Microplastics in Aquatic Environments with High Suspended Sediment Loads and Large Floating Debris
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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Water Treatment Technology

Background:

  • Microplastic (MP) pollution is a significant environmental concern, particularly from wastewater.
  • Effective MP removal technologies, like membrane filtration, require accurate quantification methods.
  • Current methods for quantifying MPs in complex water matrices can be challenging.

Purpose of the Study:

  • To demonstrate flow cytometry (FC) as an efficient technique for quantifying microplastic microbeads (MBs).
  • To evaluate the performance of microfiltration membranes in removing MPs from water.
  • To develop a model for estimating MP size using FC data.

Main Methods:

  • Plastic microbeads (1-5 μm) were suspended in water with and without humic acid.
  • Microplastic concentrations were measured using flow cytometry (FC).
  • Two microfiltration membranes (0.45 μm and 5 μm) were tested for MP rejection, with FC used for measurements.

Main Results:

  • FC accurately quantified MB concentrations under various solution conditions.
  • The 0.45 μm membrane achieved >99% MB rejection.
  • The 5 μm membrane showed variable rejection (40% to >95%) influenced by solution conditions and filtration time.

Conclusions:

  • Flow cytometry is a robust tool for quantifying microplastics in aquatic samples.
  • Membrane filtration shows promise for MP removal, with pore size being a critical factor.
  • FC-based size estimation revealed preferential passage of smaller MPs through the 5 μm membrane.