High-solids saccharification of non-pretreated citrus peels through tailored cellulase

Citrus peels, characterized by their low lignin and high sugar content, have been drawing increasing attention as a valuable lignocellulosic biomass with significant potential in biorefinery. Notably, in this study, the citrus waste was found to be enzymatically accessible without any pretreatment. Moreover, to promote the high-solids saccharification of the citrus peels, a tailored cellulase cocktail was formulated by response surface methodology (RSM), along with a fed-batch strategy aiming to obtain a high substrate loading. The study resulted in an optimized cellulase cocktail (7.08 U/g DM of β-glucosidase, 164.17 U/g DM of hemicellulase, 47.38 mg/g DM of sophorolipid, and 64.68 mg/g DM of Tween 80) and achieved solids loading of 22 % with a total sugar concentration of 123.84 g/L, corresponding to a yield of 93.12 % (65.28 % in batch operation). These findings provided essential validation for the efficient utilization of citrus waste, ensuring them promising potential as feedstock for sugar platforms.