Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peroxisomes01:24

Peroxisomes

11.1K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
11.1K
Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

86.6K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
86.6K
Subcellular Fractionation01:32

Subcellular Fractionation

6.9K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial presequences are more than just address labels.

Protein science : a publication of the Protein Society·2026
Same author

A dynamic displacement mechanism drives protein import into mitochondria.

bioRxiv : the preprint server for biology·2026
Same author

The ribosome-associated complex regulates cytosolic translation upon mitoprotein-induced stress.

The FEBS journal·2025
Same author

Conserved function, divergent evolution: mitochondrial outer membrane insertases across eukaryotes.

Biological chemistry·2025
Same author

The mammalian protein MTCH1 can function as an insertase.

Journal of cell science·2025
Same author

Mpf1 affects the dual distribution of tail-anchored proteins between mitochondria and peroxisomes.

EMBO reports·2025

Related Experiment Video

Updated: Jun 9, 2025

Purification of Mitochondria from Yeast Cells
10:39

Purification of Mitochondria from Yeast Cells

Published on: August 24, 2009

25.1K

A modified procedure for separating yeast peroxisomes from mitochondria.

Nitya Aravindan1, Doron Rapaport1

  • 1Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany.

Methods in Enzymology
|October 25, 2024
PubMed
Summary
This summary is machine-generated.

Separating mitochondria and peroxisomes in yeast is challenging due to similar densities. This study presents an optimized method using specific growth conditions and gradient centrifugation for improved organelle isolation and protein localization studies.

Keywords:
Density gradientFis1MitochondriaPeroxisomesSubcellular fractionationYeast

More Related Videos

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
14:44

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 14, 2014

12.9K
Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
07:55

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

1.3K

Related Experiment Videos

Last Updated: Jun 9, 2025

Purification of Mitochondria from Yeast Cells
10:39

Purification of Mitochondria from Yeast Cells

Published on: August 24, 2009

25.1K
Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
14:44

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 14, 2014

12.9K
Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
07:55

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Published on: June 16, 2023

1.3K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Mitochondria and peroxisomes are essential organelles with overlapping functions and shared proteins.
  • Studying dually localized proteins requires effective separation of these organelles.
  • Traditional methods struggle to separate mitochondria and peroxisomes due to similar densities in Saccharomyces cerevisiae.

Purpose of the Study:

  • To develop an optimized procedure for separating mitochondria and peroxisomes from yeast.
  • To enable the study of dually localized proteins and their unique functions in each organelle.
  • To improve the understanding of organelle biogenesis and protein localization.

Main Methods:

  • Optimizing yeast growth conditions to increase peroxisome number and density.
  • Subcellular fractionation and differential centrifugation.
  • Gradient centrifugation for enhanced organelle separation.

Main Results:

  • Achieved improved separation of mitochondria and peroxisomes.
  • Demonstrated the utility of the method for studying dual protein localization, exemplified by Fis1.
  • Facilitated the investigation of organelle-specific protein functions.

Conclusions:

  • The described method effectively separates mitochondria and peroxisomes in yeast.
  • This technique is crucial for analyzing dually localized proteins and their roles.
  • Advances the study of organelle biogenesis and function in Saccharomyces cerevisiae.