Enhancing Cellular Homeostasis: Targeted Botanical Compounds Boost Cellular Health Functions in Normal and Premature...

Area of Science:

• Dermatology
• Molecular Biology
• Pharmacology

Background:

• Human skin aging impairs function and causes visible changes.
• Baricitinib, a JAK1/2 inhibitor, has known anti-aging effects.
• Identifying botanical alternatives to baricitinib is of therapeutic interest.

Purpose of the Study:

• To identify botanical compounds that mimic baricitinib's anti-aging effects.
• To investigate the impact of these compounds on cellular aging pathways.
• To evaluate their efficacy in normal aged and premature aging skin cells.

Main Methods:

• In silico screening of a botanical compound library.
• Testing selected compounds on normal aged and Hutchinson-Gilford Progeria Syndrome (HGPS) fibroblasts.
• Assessing inhibition of the JAK/STAT pathway and cellular aging markers.

Main Results:

• Seven botanical compounds were identified and analyzed.
• These compounds inhibited the JAK/STAT pathway, reducing p-STAT1 and p-STAT3.
• Treatments improved cell proliferation, reduced senescence, enhanced autophagy, and ameliorated aging phenotypes in fibroblasts without cytotoxicity.
• Specific compounds like Resveratrol and Bisdemethoxycurcumin showed significant benefits.

Conclusions:

• Botanical compounds can effectively mitigate age-related cellular alterations in skin.
• These compounds offer potential as novel anti-aging therapies for skin health.
• Further in vivo studies are needed to confirm therapeutic applications.