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Transcriptomic Analysis of Hippocampus abdominalis Larvae Under High Temperature Stress
Wenjie Xiao1,2, Baoying Guo2, Jie Tan1
1Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Genes
|October 26, 2024
Summary
Temperature stress significantly impacts gene expression in seahorse larvae (Hippocampus abdominalis). Higher temperatures (27°C) caused mortality and altered genes involved in metabolism and signaling pathways.
Area of Science:
- Marine biology
- Genomics
- Environmental stress response
Background:
- Marine species face increasing environmental challenges.
- Understanding temperature stress impacts is crucial for conservation.
- Hippocampus abdominalis is a model organism for studying marine fish responses.
Purpose of the Study:
- To investigate the molecular mechanisms of acute temperature stress in Hippocampus abdominalis.
- To identify differentially expressed genes and pathways affected by elevated temperatures.
- To provide insights into the adaptive strategies of H. abdominalis to thermal stress.
Main Methods:
- RNA sequencing (RNA-seq) was performed on 20-day-old H. abdominalis.
- Fish were exposed to control (18°C) and treatment (21°C, 24°C, 27°C) conditions for 24 hours.
- Differential gene expression analysis was conducted using Gene Ontology and KEGG pathway enrichment.
Main Results:
- Mortality reached 50% at 27°C, while 21°C and 24°C showed no significant impact.
- 141, 333, and 1598 differentially expressed genes were identified at 21°C, 24°C, and 27°C, respectively.
- Upregulated genes were linked to the FoxO signaling pathway and mitophagy; downregulated genes were associated with steroid biosynthesis and DNA replication.
Conclusions:
- Temperature stress induces significant molecular changes in H. abdominalis.
- Key pathways involved in metabolism, biosynthesis, and stress response are affected.
- This study provides a foundation for understanding and mitigating environmental stress impacts on marine organisms.
