Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide02:44

Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide

9.9K
Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.
9.9K
Deactivation Processes: Jablonski Diagram01:25

Deactivation Processes: Jablonski Diagram

592
Luminescence, the emission of light by a substance that has absorbed energy, is a process that involves the interaction of molecules with light. The energy-level diagram, or Jablonski diagram, is a graphical representation of these interactions, illustrating the various states and transitions a molecule can undergo. In a typical Jablonski diagram, the lowest horizontal line represents the ground-state energy of the molecule, which is usually a singlet state. This state represents the energies...
592
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

1.6K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
1.6K
Photochemical Electrocyclic Reactions: Stereochemistry01:26

Photochemical Electrocyclic Reactions: Stereochemistry

1.8K
The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation
1.8K
Anoxygenic Photosynthesis01:30

Anoxygenic Photosynthesis

1
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
1

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a time-resolved singlet oxygen detection system for in vivo photodynamic therapy.

Biomedical optics express·2026
Same author

Analysis of photon transport and detectability in skin tissue through Monte Carlo simulation of photosensitization-mediated fluorescence and singlet oxygen luminescence.

Photodiagnosis and photodynamic therapy·2026
Same author

Letter: The Unresolved Issue of NSBB Confounding in Statin Research for Cirrhosis.

Alimentary pharmacology & therapeutics·2026
Same author

Serum Bile Acid Profiling Across the Full Spectrum of HBV-Related Liver Diseases in Chinese Population: Implications for Diagnosis and Treatment Assessment.

Biomedicines·2026
Same author

Clinical Implications of Discordant FIB-4 and LSM in MASLD: Integrating Evidence and Optimizing Pathways - A Commentary on the Study by Rabbat et al.

Clinical and molecular hepatology·2026
Same author

Monte Carlo analysis of light fluence rate distribution in pleural photodynamic therapy: a study of geometric and optical property effects on treatment delivery.

Journal of biomedical optics·2026

Related Experiment Video

Updated: Jun 9, 2025

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
08:04

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Published on: March 18, 2014

12.8K

Singlet Oxygen in Photodynamic Therapy.

Shengdong Cui1, Xingran Guo1, Sen Wang1

  • 1MOE Key Laboratory of Medical Optoelectronics Science and Technology, Key Laboratory of Photonics Technology of Fujian Province, School of Optoelectronics and Information Engineering, Fujian Normal University, Fuzhou 350117, China.

Pharmaceuticals (Basel, Switzerland)
|October 26, 2024
PubMed
Summary
This summary is machine-generated.

Photodynamic therapy (PDT) utilizes light, photosensitizers, and oxygen to produce singlet oxygen (¹O₂). This reactive oxygen species is crucial for oxidative damage in PDT, making ¹O₂ properties and detection key research areas.

Keywords:
detection methodsphotodynamic therapyphotosensitizersinglet oxygen

More Related Videos

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
11:04

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Published on: January 13, 2023

3.0K
Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

8.8K

Related Experiment Videos

Last Updated: Jun 9, 2025

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
08:04

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Published on: March 18, 2014

12.8K
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
11:04

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Published on: January 13, 2023

3.0K
Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

8.8K

Area of Science:

  • Biochemistry
  • Photochemistry
  • Oncology

Background:

  • Photodynamic therapy (PDT) is a clinically relevant treatment modality.
  • PDT efficacy relies on photosensitizers, light, and oxygen.
  • Singlet oxygen (¹O₂) is the primary cytotoxic agent in Type II PDT reactions.

Purpose of the Study:

  • To comprehensively review the properties of singlet oxygen (¹O₂).
  • To discuss the mechanisms of ¹O₂ production during PDT.
  • To explore methods for detecting ¹O₂ in PDT and review data for approved photosensitizers.

Main Methods:

  • Literature review of photodynamic therapy, singlet oxygen, and photosensitizer research.
  • Analysis of photochemical reactions and energy transfer processes.
  • Compilation and discussion of available ¹O₂ data for regulatory-approved photosensitizing drugs.

Main Results:

  • Singlet oxygen (¹O₂) is a highly reactive species generated through Type II photochemical reactions.
  • ¹O₂ production is integral to the photocytotoxic effects of PDT.
  • Various methods exist for detecting ¹O₂, with specific data available for approved photosensitizers.

Conclusions:

  • Singlet oxygen (¹O₂) is the critical effector molecule in Type II photodynamic therapy.
  • Understanding ¹O₂ properties, production, and detection is essential for optimizing PDT.
  • Further investigation into ¹O₂ behavior and approved photosensitizers can enhance therapeutic outcomes.