Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

3.7K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
3.7K
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

6.1K
Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
6.1K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

3
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
3
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.6K
Types of RNA01:23

Types of RNA

63.3K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.3K
Antimicrobial Proteins01:23

Antimicrobial Proteins

939
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
939

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural basis for sirtuin 2 activity and modulation: Current state and opportunities.

The Journal of biological chemistry·2025
Same author

Proceedings of the second annual dengue endgame summit: A call to action.

PLoS neglected tropical diseases·2025
Same author

The Story behind the Science: On the discovery of respiratory syncytial virus.

mBio·2025
Same author

An allosteric inhibitor of sirtuin 2 blocks hepatitis B virus covalently closed circular DNA establishment and its transcriptional activity.

Antiviral research·2024
Same author

Inhibition of SIRT2 promotes death of human cytomegalovirus-infected peripheral blood monocytes via apoptosis and necroptosis.

Antiviral research·2023
Same author

BMaps: A Web Application for Fragment-Based Drug Design and Compound Binding Evaluation.

Journal of chemical information and modeling·2023

Related Experiment Video

Updated: Jun 9, 2025

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

8.2K

Drugs Targeting Sirtuin 2 Exhibit Broad-Spectrum Anti-Infective Activity.

Thomas Shenk1,2, John L Kulp Iii3, Lillian W Chiang1

  • 1Evrys Bio, LLC, Pennsylvania Biotechnology Center, 3805 Old Easton Road, Doylestown, PA 18902, USA.

Pharmaceuticals (Basel, Switzerland)
|October 26, 2024
PubMed
Summary
This summary is machine-generated.

Host-targeted anti-infective agents that modulate human sirtuin 2 (SIRT2) show promise against diverse pathogens. These agents offer a strategy to overcome drug resistance by targeting host enzymes rather than pathogen-specific products.

Keywords:
SIRT1SIRT2allosteryanti-infectiveantiviralepigeneticshost cell metabolismhost-targeted agentssirtuin

More Related Videos

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

13.8K
A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii
09:17

A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii

Published on: January 2, 2017

14.6K

Related Experiment Videos

Last Updated: Jun 9, 2025

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates
14:32

Deacetylation Assays to Unravel the Interplay between Sirtuins SIRT2 and Specific Protein-substrates

Published on: February 27, 2016

8.2K
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

13.8K
A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii
09:17

A Robust Pneumonia Model in Immunocompetent Rodents to Evaluate Antibacterial Efficacy against S. pneumoniae, H. influenzae, K. pneumoniae, P. aeruginosa or A. baumannii

Published on: January 2, 2017

14.6K

Area of Science:

  • Biochemistry
  • Pharmacology
  • Infectious Diseases

Background:

  • Direct-acting anti-infectives target pathogen products, risking resistance.
  • Host-targeted agents offer an alternative to overcome limitations of pathogen-specific drugs.
  • Human sirtuin 2 (SIRT2) is a NAD+-dependent protein deacylase, predominantly cytoplasmic.

Purpose of the Study:

  • To review SIRT2 modulators with anti-infective activity.
  • To explore their pharmacological mechanisms as anti-infectives.
  • To identify areas for future research in advancing these agents.

Main Methods:

  • Review of literature on SIRT2 modulators and their anti-infective properties.
  • Analysis of biochemical and X-ray structural data for mechanism of action.
  • Examination of cell culture and animal model data for efficacy.

Main Results:

  • Multiple small molecules targeting SIRT2 inhibit RNA/DNA viruses and intracellular bacteria.
  • Compounds act as allosteric modulators, impacting pathogen replication at multiple levels.
  • SIRT2 modulators demonstrate efficacy in cell culture and animal models.

Conclusions:

  • SIRT2 modulators represent a promising class of host-targeted anti-infectives.
  • These agents offer a potential strategy to combat drug-resistant infections.
  • Further research is needed to advance SIRT2 modulators towards clinical application.