Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.3K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.3K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

3.0K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
3.0K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

6.9K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent...
6.9K
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

2.5K
Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
2.5K
UV–Vis Spectrum01:30

UV–Vis Spectrum

1.1K
When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar...
1.1K
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

2.3K
The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mapping Patient Complexity to Educational Needs: Proof-of-Concept for a Data-Driven Framework.

Journal of dental education·2026
Same author

Clinical and Ultrasonographic Characterization of the Transition from Hashimoto Thyroiditis to Graves' Disease: A Long-term Follow-up Study.

Endocrine·2026
Same author

Forensic DNA phenotyping: Prediction of eye and hair colour and allelic frequency estimation in the Italian population for the development of a reference dataset.

Legal medicine (Tokyo, Japan)·2026
Same author

Preliminary study of mixed traces on cigarette butts and non-self DNA transfer, persistence, prevalence and recovery in different forensic scenarios.

Legal medicine (Tokyo, Japan)·2026
Same author

Overlapping application of the forensic DNA analysis workflow to buried late-medieval human skeletal remains.

International journal of legal medicine·2026
Same author

Predictive health index: integrating body composition and heart rate variability metrics with artificial intelligence to predict chronic disease risk and specific chronic non-communicable diseases.

Hormones (Athens, Greece)·2025

Related Experiment Video

Updated: Jun 9, 2025

Visualizing Visual Adaptation
04:43

Visualizing Visual Adaptation

Published on: April 24, 2017

8.9K

Chromatic Adaption of Two Universal Composites: Spectrophotometric Analysis.

Francesca Zotti1, Francesca Ferrari2, Mattia Penazzo2

  • 1Section of Dentistry and Maxillofacial Surgery, Department of Surgical Sciences, Pediatrics and Gynecology, University of Verona, 37134 Verona, Italy.

Materials (Basel, Switzerland)
|October 26, 2024
PubMed
Summary

This study found that two composite resins, Omnichroma and Estelite Bulk-Fill Flow, exhibited similar chromatic adaptability and color stability in Class V restorations over time. Spectrophotometric analysis confirmed no significant color differences between the materials at various intervals.

Keywords:
V class restorationcolor matchingcomposite resinsspectrophotometer

More Related Videos

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.5K
Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
08:33

Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings

Published on: February 26, 2016

11.4K

Related Experiment Videos

Last Updated: Jun 9, 2025

Visualizing Visual Adaptation
04:43

Visualizing Visual Adaptation

Published on: April 24, 2017

8.9K
Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.5K
Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
08:33

Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings

Published on: February 26, 2016

11.4K

Area of Science:

  • Dental Materials Science
  • Restorative Dentistry
  • Colorimetry

Background:

  • Accurate color matching is crucial for aesthetic dental restorations.
  • Composite resins are widely used for dental restorations, but their color stability can be a concern.
  • Understanding the chromatic adaptability and color stability of different composite resins is essential for clinical success.

Purpose of the Study:

  • To evaluate and compare the chromatic adaptability and color stability of Omnichroma and Estelite Bulk-Fill Flow composite resins.
  • To assess color changes in Class V restorations made with these materials at different time points.

Main Methods:

  • Class V cavities were prepared on human molars and restored with either Omnichroma or Estelite Bulk-Fill Flow.
  • Color properties were measured using spectrophotometry at baseline, immediately after restoration, 24 hours, and after thermocycling.
  • Statistical analysis (Student's t-test, Wilcoxon-Mann-Whitney, Kruskal-Wallis, Two-way ANOVA) was performed to compare color differences (ΔE) between and within material groups over time.

Main Results:

  • No statistically significant differences in color change (ΔE) were found between Omnichroma and Estelite Bulk-Fill Flow at any time point (p > 0.05).
  • Both materials demonstrated similar chromatic adaptability, with average ΔE values exceeding 2.
  • Intra-group analysis revealed no significant color stability variations over time for either composite resin.

Conclusions:

  • Both Omnichroma and Estelite Bulk-Fill Flow exhibit comparable in vitro color matching and stability in Class V restorations.
  • The tested resin composites maintain their color over different time intervals, suggesting good color stability.
  • Despite employing different color-generating mechanisms, the two composites demonstrate similar chromatic adaptability.