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Updated: Aug 8, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
Intramammary antibiotic preparations and their withholding times
Abstract:
The test for antibiotic residues in milk carried out by the Milk Marketing Board (MMB) of England and Wales was increased in sensitivity from 0.02 iu/ml penicillin or equivalent to 0.01 iu/ml on January 1, 1986. The Veterinary Products Committee (VPC) has just completed a review of those intra-mammary antibiotic preparations which had licences of right. When assessing data for product licences the VPC decided that the concentration of antibiotic residues in milk should not exceed the concentrations listed in the 1969 Report of the Joint FAO/WHO Expert Committee on Food Additives. In this publication the level of 0.006 ppm for penicillin corresponds to 0.01 iu/ml which is the level that the MMB will also be using.
Insights
The Milk Marketing Board enhanced its antibiotic residue testing sensitivity to 0.01 iu/ml, aligning with Veterinary Products Committee standards for milk safety. This ensures milk quality by limiting penicillin residue concentrations.
Area of Science:
- Food safety analysis
- Veterinary medicine
- Dairy science
Background:
- The Milk Marketing Board (MMB) previously tested milk for antibiotic residues at 0.02 iu/ml.
- Regulatory bodies like the Veterinary Products Committee (VPC) review antibiotic preparations for safety.
- Established guidelines from the Joint FAO/WHO Expert Committee on Food Additives (1969) set acceptable residue limits.
Purpose of the Study:
- To report the increased sensitivity of the MMB's antibiotic residue testing.
- To align MMB testing levels with international food additive standards.
- To ensure the safety and quality of milk by controlling antibiotic residues.
Main Methods:
- The MMB increased the sensitivity of its penicillin residue test from 0.02 iu/ml to 0.01 iu/ml.
- The Veterinary Products Committee (VPC) reviewed intra-mammary antibiotic preparations with existing licences.
- Residue concentration assessments were based on the 1969 FAO/WHO Expert Committee report.
Main Results:
- The MMB's new testing sensitivity of 0.01 iu/ml for penicillin or equivalent was implemented on January 1, 1986.
- The VPC's review confirmed that milk residue concentrations should not exceed established FAO/WHO limits.
- The 0.006 ppm penicillin level, equivalent to 0.01 iu/ml, is the standard adopted by both the VPC and MMB.
Conclusions:
- The MMB's enhanced testing protocol ensures stricter control over antibiotic residues in milk.
- Alignment with international standards provides a consistent and reliable measure for milk safety.
- The updated testing threshold supports the production of high-quality milk free from excessive antibiotic contamination.
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