The prognostic significance of twist in pancreatic cancer and its role in cancer promotion through the regulation of the immune microenvironment and EMT mechanisms
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View abstract on PubMed
Summary
This summary is machine-generated.High Twist expression in pancreatic cancer (PAAD) indicates a poor prognosis and reduced immunotherapy effectiveness. Lower Twist levels correlate with better survival and increased anti-tumor immune cell infiltration.
Area Of Science
- Oncology
- Immunology
- Molecular Biology
Background
- Pancreatic cancer (PAAD) has a poor prognosis and low immunogenicity, limiting immunotherapy response.
- The role of Twist, a gene involved in epithelial-mesenchymal transition (EMT), in PAAD's immune microenvironment is not well understood.
- Understanding factors influencing PAAD immunity is crucial for improving treatment outcomes.
Purpose Of The Study
- To investigate the prognostic significance of Twist in pancreatic ductal adenocarcinoma (PAAD).
- To explore the relationship between Twist expression and the tumor immune microenvironment (TME) in PAAD.
- To assess the potential of Twist as a biomarker for immunotherapy response in PAAD.
Main Methods
- Analysis of transcriptional and epigenetic data for Twist in PAAD using public databases.
- Functional enrichment analysis to determine Twist's biological role.
- Utilized CIBERSORT and TIDE databases to assess Twist's association with immune cell infiltration, immune checkpoints, and immunotherapy sensitivity.
- Verified Twist expression in PAAD cell lines and patient tissues.
Main Results
- Twist mRNA expression was upregulated in PAAD and correlated with gene methylation.
- Lower Twist expression was associated with better overall survival.
- High Twist expression reduced anti-tumor immune cell infiltration and promoted an immunosuppressive TME.
- Twist expression correlated with EMT markers and predicted insensitivity to immunotherapy.
Conclusions
- Elevated Twist expression in PAAD is linked to a poor prognosis.
- Twist promotes tumor progression via EMT and suppresses the immune microenvironment, diminishing immunotherapy effectiveness.
- Twist may serve as a predictive biomarker for immunotherapy response in pancreatic cancer.
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