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Targeted no-releasing L-arginine-induced hesperetin self-assembled nanoparticles for ulcerative colitis intervention

  • 0SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Acta Biomaterialia +

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October 26, 2024

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October 26, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

New natural nanoparticles effectively scavenge reactive oxygen species (ROS) and reduce inflammation in ulcerative colitis (UC). This oral treatment targets inflamed colon regions, offering a promising therapeutic strategy for managing UC by reducing oxidative stress and repairing the intestinal barrier.

Area Of Science

  • Biomedical Nanotechnology
  • Gastroenterology
  • Materials Science

Background

  • Reactive oxygen species (ROS) overproduction drives ulcerative colitis (UC) pathogenesis.
  • A persistent ROS-inflammation cycle accelerates UC development.
  • Targeted ROS scavenging is critical for effective UC management.

Purpose Of The Study

  • To synthesize and evaluate natural, carrier-free nanoparticles (HST-Arg NPs) for UC intervention.
  • To assess the ROS-scavenging and anti-inflammatory properties of HST-Arg NPs.
  • To investigate the targeted delivery and therapeutic efficacy of HST-Arg NPs in a UC mouse model.

Main Methods

  • Synthesis of hesperetin (HST), L-arginine (L-Arg), and vanillin (VA) based nanoparticles (HST-Arg NPs) via Mannich reaction and π-π stacking.
  • Evaluation of antioxidant capabilities, gastrointestinal stability, and targeted accumulation in inflamed colonic tissue.
  • Assessment of ROS-triggered nitric oxide (NO) release and therapeutic effects in a dextran sulfate sodium (DSS)-induced UC mouse model.

Main Results

  • HST-Arg NPs demonstrated good antioxidant capacity and stability for oral delivery.
  • Negatively charged NPs selectively accumulated in positively charged inflamed colon regions.
  • In vivo studies showed significant mitigation of colonic injury, reduced pro-inflammatory cytokines, and improved intestinal barrier integrity.
  • ROS-triggered NO release was observed in the inflammatory microenvironment.

Conclusions

  • Natural carrier-free HST-Arg NPs offer a targeted and effective strategy for UC intervention.
  • The nanoparticles successfully scavenge ROS and provide gas intervention at the inflammation site.
  • This approach provides a promising foundation for developing novel oral nanoparticle formulations for inflammatory diseases.

Keywords:

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