Development and validation of a novel endoplasmic reticulum stress-related lncRNAs signature in osteosarcoma
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Researchers developed a new prognostic signature for osteosarcoma (OS) using long non-coding RNAs (lncRNAs) linked to endoplasmic reticulum stress (ERS). This signature predicts patient survival and guides targeted therapy, identifying LINC02298 as a key driver of OS progression.
Area Of Science
- Oncology
- Molecular Biology
- Bioinformatics
Background
- Osteosarcoma (OS) progression is influenced by long non-coding RNAs (lncRNAs).
- Endoplasmic reticulum stress (ERS) contributes to tumor development, but its precise role in OS is unclear.
- Identifying molecular markers for OS prognosis and treatment is crucial.
Purpose Of The Study
- To develop a prognostic signature of lncRNAs associated with endoplasmic reticulum stress (ERS) in osteosarcoma (OS).
- To predict overall survival and guide treatment strategies for OS patients.
- To investigate the functional role of specific lncRNAs and their impact on immune response and drug sensitivity.
Main Methods
- Utilized UCSC Xena database for OS transcriptional and clinical data.
- Identified differentially expressed ERS genes and correlated them with lncRNAs to define an ERLs signature using LASSO and Cox regression.
- Conducted Gene Set Variation Analysis (GSVA), assessed drug sensitivity and immune infiltration, and performed RT-qPCR and in vitro experiments for functional validation.
Main Results
- A 5-lncRNA ERLs prognostic signature (AC023157.3, AL031673.1, LINC02298, LINC02328, SNHG26) was successfully constructed and validated for OS.
- High-risk patients exhibited suppressed immune activation, reduced immune cell infiltration, and lower immunotherapy responsiveness.
- In vitro experiments confirmed LINC02298 promotes OS cell invasion, migration, and proliferation, and it is differentially expressed across multiple cancers.
Conclusions
- The novel ERLs signature accurately predicts osteosarcoma prognosis and survival.
- LINC02298 plays a significant role in promoting OS progression and serves as a potential pan-cancer biomarker.
- This signature offers new avenues for clinical prognosis prediction and establishes a basis for targeted therapy in OS.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...