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Other Glycolytic Pathways

Other Glycolytic Pathways

The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...

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Related Experiment Video

Updated: Jun 9, 2025

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

Understanding metabolic plasticity at single cell resolution.

Christina C Abbate¹, Jason Hu¹, John G Albeck¹

¹Department of Molecular and Cellular Biology, University of California, Davis, U.S.A.

Essays in Biochemistry

|October 28, 2024

Summary

This summary is machine-generated.

Cellular metabolism is not uniform, varying within tissues and cell types due to factors like cell cycle and circadian rhythms. Detecting this metabolic heterogeneity requires advanced methods such such as single-cell metabolomics.

Keywords:

adenosine triphosphate fluorescence resonance energy transfer glycolysis metabolic regulation oxidative phosphorylation systems biology

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Area of Science:

Biochemistry
Cell Biology
Metabolomics

Background:

Cellular metabolic function exhibits significant heterogeneity, extending beyond tissue-specific differences to variations within tissues and even individual cell types.
Understanding the sources and detection of this metabolic variation is crucial for advancing cell biology and disease research.

Purpose of the Study:

To explore the diverse origins of cellular metabolic heterogeneity, focusing on central carbon metabolism.
To review and compare methodologies for detecting metabolic variation at the single-cell level.
To highlight the utility of biosensors in conjunction with metabolic perturbations for revealing cellular metabolic differences.

Main Methods:

Review of literature on cellular metabolism and heterogeneity.
Discussion of single-cell metabolomics and RNA-sequencing techniques.
Focus on biosensors for AMP-activated protein kinase (AMPK) and central carbon metabolites.

Main Results:

Cellular metabolic function varies due to multiple intrinsic and extrinsic factors, including cell cycle, circadian rhythms, and metabolic cycles.
Single-cell metabolomics and RNA-sequencing are powerful tools for detecting metabolic heterogeneity.
Biosensors, particularly for AMPK and key metabolites, combined with perturbations, provide direct evidence of metabolic variance.

Conclusions:

Cellular metabolic heterogeneity is a widespread phenomenon driven by various biological rhythms and cycles.
Advanced single-cell analytical techniques are essential for characterizing metabolic differences.
Targeted biosensor development and application offer critical insights into cellular metabolic dynamics.