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Thread-Based Bienzymatic Biosensor for Linoleic Acid Detection.
Jacopo Giaretta1, Farshad Oveissi1, Sina Naficy1,2
1School of Chemical and Biomolecular Engineering, and the Centre for Advanced Food Engineering, The University of Sydney, Sydney, NSW 2006, Australia.
ACS Omega
|October 28, 2024
Summary
A novel cotton thread sensor detects linoleic acid, a key biomarker for metabolic disorders and food quality. This low-cost, portable device offers sensitive and selective nonesterified fatty acid (NEFA) detection for medical and food applications.
Area of Science:
- Biomedical Engineering
- Biosensors
- Analytical Chemistry
Background:
- Nonesterified fatty acids (NEFAs) are crucial biomarkers for metabolic disorders and food quality assessment.
- Current detection methods face challenges in portability, scalability, and cost-effectiveness.
- Early identification of NEFAs is vital for medical diagnosis and food quality control.
Purpose of the Study:
- To develop a portable and cost-effective sensor for detecting nonesterified fatty acids (NEFAs).
- To demonstrate the viability of a bienzymatic sensor using cotton thread for linoleic acid detection.
- To achieve sensitive and selective detection of linoleic acid in biological and food samples.
Main Methods:
- Fabrication of a chemiresistive sensor using cotton thread functionalized with poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS).
- Integration of enzymes horseradish peroxidase (HRP) and lipoxygenase (LOX) into the PEDOT:PSS/cotton thread matrix.
- Utilizing the specific enzymatic reaction of lipoxygenase (LOX) with linoleic acid for detection.
Main Results:
- Achieved a sensitive detection range for linoleic acid from 161 nM to 16.1 μM.
- Demonstrated exceptional selectivity for linoleic acid, even in the presence of other unsaturated fatty acids.
- The PEDOT:PSS/HRP/LOX system integrated into a single thread showed high performance.
Conclusions:
- The cotton thread-based bienzymatic sensor is a viable platform for sensitive and selective linoleic acid detection.
- This technology offers a promising low-cost, portable solution for NEFA monitoring in medical and food industries.
- The developed system can be adapted for detecting other polyunsaturated fatty acids and complex biomolecules.
