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Updated: Jun 9, 2025

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions
Published on: January 7, 2019
Odour and indoor air quality hazards in railway cars: an Australian mixed methods case study
Shaneel Chandra1, Lisa Bricknell1, Sandrine Makiela1
1College of Science and Sustainability, School of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton North, QLD 4702 Australia.
Purpose:
This case study aimed to diagnose the cause(s) of a seasonal, and objectionable odour reported by travellers and drivers in the railway cars of Australian passenger trains. The research questions were to: (1) identify whether significant microbial colonisation was present within the air handling system of trains and causing the odours; to (2) identify other potential sources and; (3) remedial options for addressing the issue.
Methods:
A mixed-methods, action research design was used adopted. Sections of the heating, ventilation, and air conditioning (HVAC) systems from odour-affected trains were swabbed for bacteria and fungi and examined for evidence of wear, fatigue and damage on-site and off-site. Insulation foam material extracted from the walls of affected trains was also subjected to a chemical assessment following exposure to varying humidity and temperature conditions in a climate simulator. This was accompanied by a qualitative sensory characterisation.
Results:
Upon exposure to a variety of simulated temperature and humidity combinations to recreate the odour, volatile chemical compounds released from the insulation foam by water were identified as its likely cause. In addition, a range of potentially serious pathogenic and odour-causing microbes were cultured from the HVAC systems, although it is considered unlikely that bacterial colonies were the odour source.
Conclusion:
The research has implications for the sanitising and maintenance policies for HVAC systems on public transport, especially when operating in humid environments. The sanitary imposition, especially in the wake of COVID-19 may be required to ensure the safety of the travelling public and drivers.
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