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  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. The Circrna Circscaf8 Promotes Tumor Growth And Metastasis Of Gastric Cancer Via Mir-1293/timp1signaling.

The circRNA circSCAF8 promotes tumor growth and metastasis of gastric cancer via miR-1293/TIMP1signaling.

Bin Mei1, Jiajie Chen2, Yang Peng3

  • 1Hepatic Surgery Centre, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Gene Therapy
|October 28, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Circular RNA SCAF8 (circSCAF8) is upregulated in gastric cancer and promotes tumor growth and metastasis. Inhibiting circSCAF8 suppressed cancer progression by increasing tissue inhibitor of metalloproteinases 1 (TIMP1) expression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Circular RNAs (circRNAs) are emerging as key regulators in various cancers, but the role of circSCAF8 in gastric cancer is largely unknown.
  • SR-like CTD-associated factor 8 (SCAF8) is involved in transcriptional termination, yet its circRNA form (circSCAF8) function requires elucidation.

Purpose of the Study:

  • To investigate the role and mechanism of circSCAF8 in gastric cancer progression.
  • To determine the relationship between circSCAF8 expression and patient survival.
  • To explore the potential of circSCAF8 as a therapeutic target.

Main Methods:

  • Quantitative real-time PCR to assess circSCAF8 expression in gastric cancer tissues and cell lines.
  • Cell proliferation, invasion, and migration assays (in vitro) and bioluminescence imaging (in vivo) to evaluate the effect of circSCAF8 knockdown.

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  • RNA immunoprecipitation (RIP) and circRNA pulldown assays to confirm molecular interactions.
  • Rescue experiments to validate the regulatory pathway.

    • Main Results:

    • circSCAF8 expression was significantly elevated in gastric cancer, correlating with lymph node metastasis and poor patient survival.
    • Knockdown of circSCAF8 using shRNA inhibited gastric cancer cell proliferation, invasion, and migration in vitro and suppressed tumor growth and lung metastasis in vivo.
    • circSCAF8 directly binds to miR-1293 but does not regulate its expression; instead, it influences the expression of TIMP1, a downstream target of miR-1293.
    • miR-1293 directly suppresses TIMP1, and circSCAF8 knockdown's inhibitory effects were reversed by TIMP1 overexpression.

    Conclusions:

    • circSCAF8 is oncogenic in gastric cancer, promoting tumor growth and metastasis.
    • circSCAF8 inhibition represents a potential therapeutic strategy for gastric cancer.
    • The circSCAF8/miR-1293/TIMP1 axis plays a critical role in gastric cancer progression.