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Updated: Jun 9, 2025
Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
Designing a novel paper-based microfluidic disc for rapid and simultaneous determination of multiple nutrient salts
Zhentao Sun1, Youquan Zhao2,1, Yameng Liu1
1School of Precision Instrument and Optoelectronic Engineering, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, People's Republic of China.
Abstract:
In the face of worsening water quality and escalating water environmental emergencies, this study developed a paper-based microfluidic disk for rapid, on-site determination of ammonia nitrogen, nitrates, nitrites, and phosphates in water. The method utilizes centrifugal microfluidics and paper-based technology, thus simplifying the operation while eliminating the need for on-site reagent preparation. Experimental results demonstrate that the disk requires only 80 microliters of a water sample and 2 minutes to complete the quantitative analysis of the four nutrients, with a coefficient of variation below 1.72% and spike recoveries ranging from 92% to 113%. The development of the disk provides an effective and rapid, on-site testing tool for water quality analysis.
