Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stereotactic Accelerated Partial Breast Irradiation Using CyberKnife with Non-Invasive Skin Fiducial Marker Tracking in Early-Stage Breast Cancer: A Retrospective Study of Feasibility, Dosimetry, and Early Outcomes.

Breast cancer (Dove Medical Press)·2025
Same author

Transcriptomic and enzymatic analysis of peroxidase families at the early growth stage of halophyte ice plant (Mesembryanthemum crystallinum L.) under salt stress.

Botanical studies·2025
Same author

Stereotactic body radiotherapy using CyberKnife versus interstitial brachytherapy in accelerated partial breast irradiation on left-sided breast: A comparison of dosimetric characteristics and preliminary clinical results.

Breast (Edinburgh, Scotland)·2024
Same author

The phosphorylation of carboxyl-terminal eIF2α by SPA kinases contributes to enhanced translation efficiency during photomorphogenesis.

Nature communications·2024
Same author

COP1-ERF1-SCE1 regulatory module fine-tunes stress response under light-dark cycle in Arabidopsis.

Plant, cell & environment·2024
Same author

Phytochrome Signaling Networks.

Annual review of plant biology·2021
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Jun 9, 2025

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
08:39

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

1.5K

Translation Efficiency Test Using Polysome Profiles Under Heat Stress.

Hui-Hsien Chang1, Mei-Chun Cheng2

  • 1Department of Biochemical Science and Technology, National Taiwan University.

Journal of Visualized Experiments : Jove
|October 28, 2024
PubMed
Summary
This summary is machine-generated.

This study details a method to measure gene translation efficiency in plants under heat stress using polysome profiling. This helps understand plant resilience and develop climate-change-ready crops.

More Related Videos

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
10:59

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Published on: May 19, 2014

18.2K
Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
10:00

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

28.2K

Related Experiment Videos

Last Updated: Jun 9, 2025

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
08:39

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

1.5K
Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
10:59

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Published on: May 19, 2014

18.2K
Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
10:00

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

28.2K

Area of Science:

  • Plant molecular biology
  • Environmental stress response
  • Gene expression regulation

Background:

  • Translational control is vital for plant adaptation to environmental changes like heat stress.
  • Understanding gene translational activity under stress is key to improving crop resilience.
  • Global climate change necessitates enhanced crop stress tolerance.

Purpose of the Study:

  • To present a detailed methodology for assessing translation efficiency in plants under heat stress.
  • To provide a protocol for analyzing translational regulation in response to heat stress.
  • To aid in developing crops with improved heat tolerance.

Main Methods:

  • Heat stress treatment of Arabidopsis plants under controlled conditions.
  • Polysome profiling via sucrose gradient centrifugation to assess ribosome loading on mRNAs.
  • Isolation of polysomal and non-polysomal RNA, with spike-in RNA for quantification.
  • Calculation of translation efficiency by comparing RNA distribution and qRT-PCR analysis.

Main Results:

  • The methodology allows for the assessment of translational control mechanisms under heat stress.
  • It enables the examination of ribosome occupancy on specific gene mRNAs.
  • Provides a quantitative measure of translation efficiency changes due to heat stress.

Conclusions:

  • This protocol offers a robust approach to analyze heat stress-induced translational regulation in plants.
  • It contributes to understanding plant molecular mechanisms for environmental adaptation.
  • The findings support the development of strategies for enhancing crop heat tolerance.