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Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
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Response to El-Dallal et al

Thomas Ka-Luen Lui1,2, Wai K Leung1,2

  • 1Department of Medicine, Li Ka Shing Faculty of Medicine, School of Clinical Medicine, University of Hong Kong, Hong Kong, China.

The American Journal of Gastroenterology
|October 28, 2024
PubMed
Summary

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.