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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Updated: Jun 23, 2026

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Published on: August 15, 2012

Growing microglia in the lab.

Jonathan M Levenson¹, Hio Tong Kam², Dong Feng Chen²

¹FireCyte Therapeutics, Inc, Boston, United States.

|October 28, 2024

Summary

This summary is machine-generated.

Human stem cell-derived microglia transplantation offers new therapeutic avenues for neurodegenerative eye diseases. This research explores novel treatment strategies for conditions affecting vision.

Keywords:

Microglia NaIO3 PLX-5622 RPE human iPSC mouse regenerative medicine retinal transplantation stem cells

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Obtaining Human Microglia from Adult Human Brain Tissue

Published on: August 30, 2020

Related Experiment Videos

Last Updated: Jun 23, 2026

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Published on: August 15, 2012

Culturing Microglia from the Neonatal and Adult Central Nervous System

Culturing Microglia from the Neonatal and Adult Central Nervous System

Published on: August 9, 2013

Obtaining Human Microglia from Adult Human Brain Tissue

Obtaining Human Microglia from Adult Human Brain Tissue

Published on: August 30, 2020

Area of Science:

Neuroscience
Ophthalmology
Stem Cell Biology

Background:

Neurodegenerative eye diseases, such as glaucoma and age-related macular degeneration, involve progressive vision loss.
Microglia, the resident immune cells of the central nervous system, play a critical role in retinal health and disease.
Current treatments for these conditions are limited, necessitating the development of novel therapeutic approaches.

Purpose of the Study:

To investigate the potential of transplanting human stem cell-derived microglia as a treatment for neurodegenerative eye diseases.
To assess the survival, integration, and functional effects of transplanted microglia in a mouse model.

Main Methods:

Human induced pluripotent stem cells (iPSCs) were differentiated into microglia-like cells.
These cells were transplanted into the eyes of mice with induced neurodegenerative conditions.
Engraftment, survival, and functional outcomes were evaluated using histological and behavioral analyses.

Main Results:

Successfully transplanted microglia survived and integrated into the host retinal environment.
Evidence of functional recovery and reduction in disease pathology was observed in treated mice.
Transplanted microglia demonstrated phagocytic activity and modulated inflammatory responses.

Conclusions:

Transplantation of human stem cell-derived microglia is a promising strategy for treating neurodegenerative eye diseases.
This approach holds potential for restoring vision and halting disease progression.
Further research is warranted to optimize cell delivery and assess long-term efficacy and safety.