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Updated: Jun 9, 2025

Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

33.2K

SF3B4 Regulates Cellular Senescence and Suppresses Therapy-induced Senescence of Cancer Cells

Seungyeon Yang1,2, Minbeom Ko1,2, Soojung Claire Hur3

  • 1Department of Biochemistry, Institute for Aging and Metabolic Diseases, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

Cancer Genomics & Proteomics
|October 28, 2024
PubMed
Abstract

Insights

Splicing factor SF3B4 regulates cellular senescence. Its reduction induces senescence, while its overexpression mitigates therapy-induced senescence (TIS) in cancer cells, suggesting SF3B4 as a therapeutic target for cancer treatment.

Area of Science:

  • Cellular biology
  • Cancer research
  • Molecular oncology

Background:

  • Cellular senescence is a permanent cell cycle exit that prevents tumor growth but contributes to aging and inflammation.
  • Therapy-induced senescence (TIS) halts cancer cell proliferation and metastasis, offering a therapeutic strategy with minimized cytotoxicity.
  • Understanding the regulators of senescence is crucial for developing effective cancer treatments.

Purpose of the Study:

  • To investigate the role of splicing factor 3B subunit 4 (SF3B4) in regulating cellular senescence.
  • To determine the involvement of SF3B4 in therapy-induced senescence (TIS) in cancer cells.
  • To explore SF3B4 as a potential therapeutic target for cancer treatment.

Main Methods:

  • Senescence induction was assessed using beta-galactosidase staining.
  • SF3B4 and p21 expression levels were quantified via RT-qPCR and western blotting.
  • Cell proliferation and cell death assays were performed to evaluate functional outcomes.

Main Results:

  • SF3B4 expression was found to decrease in senescent human fibroblasts.
  • Knockdown of SF3B4 induced senescence through a p21-dependent pathway in both fibroblasts and A549 non-small cell lung cancer (NSCLC) cells.
  • Overexpression of SF3B4 attenuated doxorubicin-induced senescence in A549 NSCLC cells.

Conclusions:

  • SF3B4 plays a significant role in regulating cellular senescence.
  • The findings suggest SF3B4 as a potential therapeutic target for enhancing cancer treatment efficacy through TIS.
  • Targeting SF3B4 could offer a novel strategy to suppress tumor growth and improve treatment outcomes.
Keywords:
NSCLCSF3B4Senescencep21therapy-induced senescence

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