Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.1K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

9.8K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
9.8K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

3
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
3

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Ion Imaging Native Mass Spectrometry: Unraveling the Structural Features and Dissociation Energetics of Macromolecular Assemblies.

Journal of the American Society for Mass Spectrometry·2026
Same author

Spatiotemporal Mapping of Drug Incorporation in Human Nails by MALDI-FTICR-MSI.

Journal of the American Society for Mass Spectrometry·2026
Same author

Isotope Decluttering Reduces Spectral Complexity while Maintaining Protein Structure.

Analytical chemistry·2026
Same author

Mass spectrometry imaging in spatial biology of pancreatic cancer.

The Analyst·2026
Same author

Ontogeny independent expression of LPCAT2 in granuloma macrophages during experimental visceral leishmaniasis.

Communications biology·2026
Same author

Integrating Ion Beam Control into a Commercial Platform for Improved Multimodal SIMS/MALDI Imaging.

Journal of the American Society for Mass Spectrometry·2026

Related Experiment Video

Updated: Jun 9, 2025

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

11.4K

Improvements in Fast Mass Microscopy for Large-Area Samples.

Edith Sandström1, Pascal Huysmans2, Frans Giskes1

  • 1The Maastricht MultiModal Molecular Imaging Institute (M4i), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht 6229 ER, The Netherlands.

Analytical Chemistry
|October 28, 2024
PubMed
Summary
This summary is machine-generated.

Fast mass microscopy (FMM) now achieves 8.5x faster imaging speeds, enabling large-area chemical analysis at high resolution. This advancement overcomes previous throughput limitations in mass spectrometry imaging.

More Related Videos

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

8.3K
Author Spotlight: Introduction to Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays
05:04

Author Spotlight: Introduction to Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays

Published on: June 13, 2023

1.4K

Related Experiment Videos

Last Updated: Jun 9, 2025

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

11.4K
Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
07:53

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

8.3K
Author Spotlight: Introduction to Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays
05:04

Author Spotlight: Introduction to Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays

Published on: June 13, 2023

1.4K

Area of Science:

  • Analytical Chemistry
  • Chemical Imaging
  • Microscopy

Background:

  • Mass spectrometry imaging (MSI) analyzes chemical composition and spatial distribution of analytes.
  • Microprobe mode MSI links spatial resolution, imaging area, and speed, limiting throughput.
  • Fast mass microscopy (FMM) decouples these parameters for improved performance.

Purpose of the Study:

  • To enhance the speed and capabilities of fast mass microscopy (FMM).
  • To enable high-resolution, large-area chemical imaging.
  • To assess FMM's performance with instrument modifications.

Main Methods:

  • Implemented instrument modifications to an FMM system, including linear encoders.
  • Collected mass spectra for each pixel to create mass images.
  • Determined the tolerance of FMM to sample height variations.

Main Results:

  • Achieved approximately 8.5x faster imaging speeds compared to previous FMM systems.
  • Enabled imaging of a 42.5 × 26 mm² sample area at 1 μm pixel size in under 4.5 minutes.
  • Demonstrated that FMM tolerates height variations of at least 218 ± 0.03 μm.

Conclusions:

  • Instrument modifications significantly enhance FMM's throughput and applicability to large samples.
  • FMM offers a powerful solution for high-resolution, rapid chemical imaging.
  • The technique is robust to sample surface variations, broadening its potential applications.