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Wipe-rinse technique for quantitating microbial contamination on large surfaces.

L E Kirschner, J R Puleo

    Applied and Environmental Microbiology
    |September 1, 1979
    PubMed
    Summary
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    An improved polyester-bonded cloth (PBC) wipe-rinse technique offers higher bioassay recovery efficiency for large spacecraft surfaces compared to traditional cotton swabs. This method is most effective for areas up to 0.74 m2.

    Area of Science:

    • Microbiology
    • Spacecraft Contamination Control
    • Analytical Chemistry

    Background:

    • Current spacecraft bioassays rely on cotton swab-rinse techniques, which have known limitations for large surface areas.
    • Assessing microbial contamination on spacecraft is critical for mission success and planetary protection.

    Purpose of the Study:

    • To evaluate an improved wipe-rinse technique for bioassaying large spacecraft surfaces.
    • To compare the efficiency of a polyester-bonded cloth (PBC) against traditional cotton swabs.

    Main Methods:

    • Four types of contamination control cloths were tested.
    • A polyester-bonded cloth (PBC) was selected based on efficiency and handling.
    • Comparative tests were conducted on simulated spacecraft surfaces using PBC and cotton swabs.

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    Main Results:

    • The polyester-bonded cloth (PBC) demonstrated significantly higher recovery efficiency (90.4%) compared to cotton swabs (75.2%).
    • PBC was most effective for sampling areas up to 0.74 m2 (8.0 ft2).

    Conclusions:

    • The polyester-bonded cloth (PBC) wipe-rinse technique is a superior method for spacecraft bioassays.
    • This improved technique enhances the detection of microbial contamination on large surfaces.