Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Artificial Intelligence for Automated Current Procedural Terminology Coding in Plastic and Reconstructive Surgery Using a Fine-tuned Hybrid Large Language Model.

Plastic and reconstructive surgery. Global open·2026
Same author

Risk Factors for the Recurrence of Macular Holes After 25-Gauge Pars Plana Vitrectomy.

Ophthalmic surgery, lasers & imaging retina·2026
Same author

A guest-host hydrogel for neural tissue engineering applications.

Journal of materials chemistry. B·2026
Same author

Screening for Periodontitis Using Blood Biomarkers and Demographic Data: A Machine Learning Study.

Oral health & preventive dentistry·2026
Same author

Co-Design of strategies to enhance access to Virtual Urgent Care models by equity-deserving populations.

PLOS digital health·2026
Same author

On-site microRNA detection with 'off-the-shelf' glucose meter empowered by chimeric probe connecting CRISPR/Cas13a activation to kinases-driven glucose phosphorylation.

Biosensors & bioelectronics·2026

Related Experiment Video

Updated: Jun 9, 2025

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.6K

Squeezable Hydrogel Microparticles for Single Extracellular Vesicle Protein Profiling.

Yoon Ho Roh1, Renee-Tyler T Morales2, Emily Huynh2

  • 1Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|October 29, 2024
PubMed
Summary

This study introduces a new method using squeezable hydrogel microparticles for ultra-sensitive, multiplexed analysis of single extracellular vesicles (EVs). This technique enhances molecular diagnostics by overcoming limitations in detecting rare EV protein expression.

Keywords:
digital assaysextracellular vesiclesmultiplexingrolling circle amplificationsqueezable hydrogel

More Related Videos

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages
08:34

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

Published on: June 30, 2023

2.0K
Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
02:36

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

903

Related Experiment Videos

Last Updated: Jun 9, 2025

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry
12:27

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

4.6K
Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages
08:34

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

Published on: June 30, 2023

2.0K
Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation
02:36

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

903

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Nanotechnology

Background:

  • Extracellular vesicles (EVs) are crucial for molecular diagnostics.
  • Current EV analysis faces challenges due to low abundance and heterogeneous protein expression.
  • High-sensitivity, multiplexed, and high-throughput single EV profiling is needed.

Purpose of the Study:

  • To develop a novel platform for ultra-sensitive and multiplexed single EV protein analysis.
  • To overcome limitations of current single EV profiling methods.
  • To enable detailed analysis of EV heterogeneity.

Main Methods:

  • Utilized squeezable methacrylated hyaluronic acid hydrogel microparticles (MHPs) as a scaffold for EV immobilization.
  • Integrated rolling circle amplification (RCA) for signal amplification.
  • Employed droplet microfluidics for high-throughput EV preparation and antibody-oligonucleotide conjugate labeling within MHPs.

Main Results:

  • Achieved ultra-sensitive detection of single EV proteins through integrated RCA.
  • Demonstrated multiplexed analysis of EV protein markers without steric hindrance.
  • Physically squeezed MHPs to align amplified signals for low-magnification visualization.
  • Successfully profiled single EV heterogeneity of cancer cell markers across different cell lines.

Conclusions:

  • Squeezable MHPs offer a robust, scalable platform for single EV analysis.
  • The developed method significantly advances molecular assay technologies for resolving EV heterogeneity.
  • This approach holds promise for improved molecular diagnostics and understanding EV biology.