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External ATP permeabilizes transformed cells to macromolecules.

M J Otero, L Carrasco

    Biochemical and Biophysical Research Communications
    |January 29, 1986
    PubMed
    Summary

    External ATP and specific ionic conditions enable transformed cells to uptake hygromycin B. This method allows selective protein entry, like alpha-sarcin, into 3T6 cells, enhanced by ATP synthesis blockers.

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    Area of Science:

    • Cell biology
    • Molecular biology
    • Biochemistry

    Background:

    • Cellular permeability is crucial for drug delivery and molecular uptake.
    • Transformed cells exhibit altered properties compared to normal cells.
    • Adenosine triphosphate (ATP) plays a vital role in cellular processes.

    Purpose of the Study:

    • To investigate the selective permeability of transformed cells to external molecules.
    • To explore the role of external ATP in enhancing cellular uptake.
    • To identify conditions that facilitate the entry of proteins into specific cell types.

    Main Methods:

    • Utilizing external adenosine triphosphate (ATP) under specific ionic conditions to induce cell permeability.
    • Employing the translation inhibitor hygromycin B to assess cell membrane permeability.
    • Comparing the uptake of proteins, such as alpha-sarcin, horseradish peroxidase, and luciferase, into 3T6 versus 3T3 cells.
    • Investigating the effect of ATP synthesis blockers (e.g., CCCP) on protein entry.

    Main Results:

    • External ATP, under defined ionic conditions, renders transformed cells permeable to hygromycin B.
    • The protein toxin alpha-sarcin demonstrates selective penetration into 3T6 cells compared to 3T3 cells.
    • Cellular entry of alpha-sarcin is enhanced by the presence of ATP synthesis blockers like CCCP.
    • Other proteins, including horseradish peroxidase and luciferase, also exhibit selective entry into 3T6 cells under these permeabilization conditions.

    Conclusions:

    • External ATP can be used to selectively permeabilize transformed cells for molecular entry.
    • 3T6 cells show enhanced permeability to specific proteins compared to 3T3 cells under these conditions.
    • This method offers a potential strategy for targeted delivery of macromolecules into specific cell types.

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