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Shotgun Lipidomics of Rodent Tissues
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Adapting lipidomic sample processing methods for boars housed in commercial settings.

Kayla M Mills1, Amanda M Minton2, Christina R Ferreira3

  • 1US Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center (BARC), Beltsville, MD 20705, USA.

Translational Animal Science
|October 30, 2024
PubMed
Summary
This summary is machine-generated.

This study developed a non-freezing method for boar semen lipidomic analysis using multiple reaction monitoring (MRM) profiling. This technique identifies fertility markers in boar ejaculate, improving breeding program efficiency.

Keywords:
boarfertilitylipidome

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Area of Science:

  • Animal Science
  • Biochemistry
  • Reproductive Biology

Background:

  • Multiple reaction monitoring (MRM) profiling is a sensitive lipid screening method for gilt fertility but not yet applied to boars.
  • Identifying boar fertility markers is crucial to prevent economic losses from subfertile animals in breeding programs.
  • Current semen cryopreservation methods for lipidomic analysis are impractical for commercial boar studs.

Purpose of the Study:

  • To develop a novel sample processing method for MRM profiling that preserves boar semen without freezing.
  • To evaluate the ejaculate lipidome of commercial boars using this new method.
  • To identify potential lipid markers associated with boar fertility and sperm metabolism.

Main Methods:

  • Semen samples from five boars were collected and immediately aliquoted into methanol to halt metabolic activity.
  • Lipids were extracted using the Bligh and Dyer method, followed by MRM profiling for lipid screening.
  • Statistical analyses, including hierarchical cluster and principal component analysis, were performed on lipidomic data.

Main Results:

  • A total of 329 lipid-related MRMs were detected, with plasma membrane lipids (74%) dominating the ejaculate lipidome.
  • Phosphatidylcholines, ceramides, phosphatidylethanolamines, and phosphatidylserines were the most abundant lipid classes.
  • Acylcarnitines (8%) were identified, with one boar showing unique abundance linked to progressive motility and sperm metabolism.

Conclusions:

  • The developed methanol-based sample processing method is suitable for MRM profiling of boar ejaculates in commercial settings.
  • This approach can identify distinct ejaculate lipidome profiles in boars, potentially indicating fertility status.
  • The method holds promise for broader applications in livestock species and commercial environments for fertility assessment.