An integrative phylogeography for inferring cryptic speciation in the Alpheus lottini species complex, an important...

We use molecular analyses, color patterns, and records of distribution of mating pairs to reconstruct the global phylogeography of Alpheus lottini, a complex of cryptic coral-associated snapping shrimp species. Molecular data support the delineation of ancestral clades A, B, and C, and suggest five additional subdivisions within clades A and B. Clades A, B1, B2, and C exhibit color pattern differences and/or evidence of assortative mating, and thus merit species-level recognition. There is no evidence for assortative mating within clades A and B1, with likely reproductive compatibility (i.e., fertile clutches) in areas of sympatry. The clade diversity peaks in the Mariana Islands and the early branching clade C is restricted to the northern periphery of the Central and Western Pacific suggesting a Pacific origin of this group outside of the Coral Triangle. These findings underscore the prevalence of allopatric processes with possible ecological or microallopatric speciation in areas where clades overlap.