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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
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  9. Predictive And Prognostic Markers
  11. Peptide-based Turn-on Fluorescent Probes For Highly Specific Detection Of Survivin Protein In The Cancer Cells.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Peptide-based Turn-on Fluorescent Probes For Highly Specific Detection Of Survivin Protein In The Cancer Cells.

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Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells.

Takeshi Fuchigami1, Tomoe Nakayama2, Yusuke Miyanari3

  • 1Laboratory of Clinical Analytical Sciences, Graduate School of Medical Sciences, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.

Chemical & Biomedical Imaging
|October 30, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers developed novel survivin-sensitive fluorescent probes (SSFPs) for cancer diagnosis. These probes utilize Förster resonance energy transfer (FRET) to detect survivin protein, showing promise for targeted cancer imaging and treatment strategies.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Survivin is a key protein overexpressed in many human cancers.
  • Targeting survivin offers a promising avenue for cancer diagnosis and therapy.
  • Developing sensitive probes for survivin detection is crucial for clinical applications.

Purpose of the Study:

  • To design and synthesize novel survivin-sensitive fluorescent probes (SSFPs).
  • To evaluate the binding affinity and fluorescence response of SSFPs to survivin.
  • To demonstrate the efficacy of SSFPs for imaging survivin in cancer cells.

Main Methods:

  • Peptide probes (Bor65-75 and survivin protein segment) were conjugated with a fluorophore (FAM) and quencher (DABCYL).
  • Förster resonance energy transfer (FRET) was employed to create a fluorescence-based detection system.
  • Binding assays with recombinant human survivin (rSurvivin) and confocal fluorescence imaging were performed.

    • Main Results:

    • SSFPs exhibited moderate to high affinity for survivin (Kd = 121-1740 nM).
    • A dose-dependent increase in fluorescence intensity was observed in the presence of rSurvivin.
    • The SSFP5 probe showed clear fluorescence in survivin-positive cancer cells (MDA-MB-231) but not in survivin-negative cells (MCF-10A).

    Conclusions:

    • The developed SSFPs function as effective survivin-specific FRET imaging probes.
    • These probes demonstrate potential for sensitive and specific detection of survivin in cancer cells.
    • SSFPs hold promise for advancing cancer diagnosis and targeted therapeutic strategies.