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Updated: Jun 9, 2025
Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
Published on: October 17, 2016
Quantitative estimation of optical properties in bilayer media within the subdiffusive regime using a tilted
Philippe De Tillieux1,2, Maxime Baillot1,3, Pierre Marquet1,2,3,4,5
1Cervo Brain Research Centre, Québec, Canada.
Significance:
As biological tissues are highly heterogeneous, there is a great interest in developing non-invasive optical approaches capable of characterizing them in a very localized manner. Obtaining accurate absolute values of the local optical properties from the measured reflectance requires finding a probe geometry, which allows us to solve this inverse problem robustly and reliably despite neglecting the higher-order moments of the scattering phase function.
Aim:
Our goal is to develop a theoretical framework for designing tilted-fiber diffuse reflectance probes that allow quantitative estimation of the optical properties corresponding to limited tissue volume (typically a few cubic millimeters).
Approach:
Relationships among probe geometry, sampled tissue volume, and robustness of the inverse solver to calculate optical properties from reflectance are studied using Monte Carlo simulations.
Results:
The analysis of the number of scattering events of the collected photons leads to the establishment of relationships among the probe geometry, the sampled tissue volume, and the validity of a subdiffusive regime for the reflectance.
Conclusions:
A methodology is proposed for the design of new compact probes with tilted fiber geometry that can quantitatively estimate the values of the optical coefficients in a localized manner within living biological tissues by recording diffuse reflectance spectra.
