Related Experiment Video
Updated: Jun 9, 2025

06:31
Precision Implementation of Minimal Erythema Dose MED Testing to Assess Individual Variation in Human Inflammatory Response
Published on: October 3, 2019
8.6K
The Effect of Positioning Guide Color on Shade Measurements Using a Clinical Spectrophotometer
Joao Arthur Dumke1, Lauren Arrua Fantine2, Suzana Nogueira3
1Post-Graduate Program in Oral Science, Division of Restorative Dentistry, Federal University of Santa Maria (UFSM).
Brazilian Dental Journal
|October 30, 2024
Summary
The color of dental positioning guides significantly impacts shade measurements on ceramic, resin composite, and bovine tooth surfaces. Guide color choice is crucial for accurate spectrophotometer readings in dental shade assessment.
Area of Science:
- Dental Materials Science
- Color Science in Dentistry
- Spectrophotometry
Background:
- Accurate shade selection is critical in restorative dentistry for achieving aesthetic outcomes.
- Clinical spectrophotometers are widely used for objective color measurements.
- The influence of ancillary devices, such as positioning guides, on measurement accuracy requires investigation.
Purpose of the Study:
- To evaluate how different colors of positioning guides affect CIEL*a*b* color coordinates of dental materials and natural tooth surfaces.
- To determine if positioning guide color influences the accuracy of spectrophotometric shade measurements.
Main Methods:
- Ten positioning guides in various colors (translucent, purple, yellow, green, blue) were fabricated for ceramic, resin composite, and bovine tooth surfaces.
- CIEL*a*b* color coordinates were measured with and without positioning guides using a clinical spectrophotometer.
- Color differences (ΔL*, Δa*, Δb*, ΔE*ab, ΔE00) were calculated and analyzed using ANOVA and Tukey's post-hoc test.
Main Results:
- Positioning guide color significantly altered color coordinates across all tested surfaces (ceramic, resin composite, bovine tooth).
- Mean color difference values (ΔE*ab, ΔE00) generally exceeded visual acceptability thresholds for all guide colors and surfaces.
- An exception was noted for the bovine tooth surface with the green positioning guide, where differences were within acceptable limits.
Conclusions:
- The color of positioning guides demonstrably affects spectrophotometric shade measurements.
- Careful selection of positioning guide color is necessary to minimize measurement bias and ensure shade accuracy in clinical dental applications.
- Current positioning guide colors, except for green on bovine teeth, introduce unacceptable color variations.
Related Concept Videos
Spectrophotometry: Introduction
3.0K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
3.0K
Indicators
48.1K
Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH of a solution, are...
48.1K
UV–Vis Spectrometers
1.3K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.3K
Instrument Calibration
152
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
152

