First comparative genomics analysis of Corynebacterium auriscanis
Ana Lua de Oliveira Vinhal1, Max Roberto Batista de Araújo1,2, Evandro Bento Rodrigues1,2
1Universidade Federal de Minas Gerais, Departamento de Genética, Ecologia e Evolução, Belo Horizonte, MG, Brasil.
Corynebacterium auriscanis, a dog pathogen, lacks true virulence factors, suggesting an opportunistic lifestyle. Genomic analysis identified 42 niche factors, including one potentially conferring rifampin resistance.
Area of Science:
- Bacteriology
- Comparative Genomics
- Pathogenomics
Background:
- Corynebacterium auriscanis is frequently isolated from dogs with otitis and dermatitis.
- It is a zoonotic pathogen transmitted via dog bites.
- Its pathogenicity mechanisms remain poorly understood.
Purpose of the Study:
- To compare genomes of C. auriscanis.
- To identify genes associated with virulence and lifestyle.
Main Methods:
- Genome sequencing of strain 32 using Illumina HiSeq 2500.
- Assembly using Unicycler and inclusion of two public genomes.
- Annotation and analysis for mobile elements, CRISPR-Cas, and virulence/resistance genes.
Main Results:
- Identified 42 factors, classified as niche factors, not true virulence factors.
- The gene rbpA may provide basal rifampin resistance.
- Comparison with co-isolated pathogens was performed.
Conclusions:
- Absence of true virulence factors supports an opportunistic pathogen lifestyle for C. auriscanis.
- Genomic insights into C. auriscanis lifestyle and potential resistance mechanisms were gained.
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