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Updated: Jul 1, 2026
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
A smartphone-assisted ultrasensitive colorimetric aptasensor based on DNA-encoded porous MXene nanozyme for visual
Jingtian Chi1, Peng Ju2, Fan Bi3
1College of Chemistry and Chemical Engineering, Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, No. 238 Songling Road, Qingdao 266100, PR China; Observation and Research Station of Bohai Eco-Corridor and Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao 266061, PR China.
Abstract:
The smartphone-assisted ultrasensitive colorimetric aptasensor based on DNA-encoded porous Ti3C2 nanozyme (Apt-P-Ti3C2) was exploited for real-time detection of OA. Porous Ti3C2 (P-Ti3C2) MXene with outstanding peroxidase-like activities were crafted using microwave combustion, facilitating the efficient catalysis of chromogenic substrate oxidation by H2O2. The integration of a considerable number of unsaturated Ti center edges and residual Mn2+ within the single-layer porous Ti3C2 framework augmented the adsorption capacity of DNA aptamer, thereby yielding a heightened catalytic efficacy of P-Ti3C2 nanoparticles. The enhanced catalytic activity of P-Ti3C2 can be partially diminished through specific recognition of OA. Concurrently, a smartphone platform was integrated for signal reading based on the colorimetric sensing strategy. The smartphone-based biosensor exhibited a reliable and ultrasensitive capability for OA detection with the detection limit of 0.38 ng·mL-1. It is anticipated that the developed smartphone-based biosensing platform can provide a prospective ultrasensitive detection method for marine algal toxin in food analysis.
